Optimization of SSR-PCR Reaction System and Selection for Polymorphic Primers in Chili Pepper(Capsicum annuum var.longunt)
In the study,the factors affecting the SSR-PCR results of pepper were studied with L9(34) orthogonal design,and the polymorphism of 420 primer pairs were screened by yellow pepper fruit mutant HJ2002 and high generation inbred 0599-1.The results showed that optimal reaction system was the total 20 μL reaction system containing 20 ng of DNA template,100 μmol · L-1 dNTP,0.6 μmol · L-1 primer,2.5 mmol · L-1 Mg2+ and 1.5 μmol of Taq DNA polymerase enzyme.All the 420 primer pairs were selected at random for yielding amplification products,of which 72 primer pairs showed polymorphism between two different pepper varieties.The polymorphism rate was 17.1％ while the average alleles per locus was 2.67.This optimized SSR-PCR system and polymorphism primer could be applied for further research on germplasm analysis,molecular marker-assisted breeding,construction of molecular genetic linkage maps and gene cloning in chili pepper.
NETL
NSTL
万方数据
维普
