Establishment of DNA Fingerprinting and Analysis of Genetic Diversity among Prunus aviun Cultivars
In order to identify the cultivars of Prunus aviun and analyze the genetic relationship among different cultivars at a molecular level,we constructed the digital fingerprint and model fingerprint (ClusterProject)for 40 common Prunus aviun cultivars using EST-SSR(Expressed Sequence Tag-Simple Sequence Repeats) molecular markers.The 24 pairs of primers amplified a total of 129 alleles including 118 polymorphic alleles with a polymorphism rate of 91.2％.Each pairs of primers can amplify 3-10 alleles,with an average of 5.38 alleles per locus.The length of amplified products was 100-500 bp,with the mean value of PIC(Polymorphism information content) being 0.654,Nm (Gene flow)being 1.402,and the mean value of H.(Heterozygosity) being 0.495.Among the 24 primers,8 primers can be used as a preferred choice for fingerprinting,namely PA17,PA32,PA51,PA54,PA73,PA99,PA101 and PA202,in which 5 primers(PA17,PA51,PA99,PA101 and PA202) can be employed in combination to identify the 40 cultivars.Cluster analysis showed that the 40 cultivars can be divided into five groups at a genetic distance of 0.71;the genetic relationship as indicated by the cluster completely reflected the characteristics and the maturation stage of the cultivars.
NETL
NSTL
万方数据
维普
