Transcriptomic Changes of Postharvest Cucumber during Cold Acclimation
Postharvest cucumber is a kind of cold-sensitive fruit vegetable,which is subjected to chill-ing injury during cold storage.The results of previous studies showed that cold acclimation treatment could induce the chilling tolerance of postharvest cucumber and reduce the occurrence of chilling inju-ry.In order to explore the transcriptomic changes induced by cold acclimation,transcriptomic changes during cold acclimation were analyzed.Compared with that before storage(0 h),cold acclimation sig-nificantly affected the differential expression of 1 870 and 3 550 genes at 12 h and 72 h,respectively.The expression verification results of representative differentially expressed genes(DEGs)showed that the results obtained by qRT-PCR and RNA-seq were consistent in general,which conformed the accuracy and reliability of RNA-seq data.The results of GO enrichment analysis showed that the DEGs induced by cold acclimation were mainly enriched in three GO pathways:oxidation-reduction process,integral component of membrane and transcription factor activity,suggesting that cold accli-mation enhanced the chilling tolerance of cucumber fruit by regulating oxidation-reduction status and cell membrane component.Further analysis showed that 104 transcription factor genes were respon-ded to cold acclimation,and differentially expressed transcription factors were mainly ERF,bZIP,WRKY and HSF families,indicating that transcription regulation mediated by transcription factor played an important role in cold-acclimation-induced chilling tolerance in cucumber fruit.The results provide new insights into the induction of chilling tolerance in harvest cucumbers,and help to deepen the understanding of the molecular mechanisms of chilling tolerance induced by cold acclimation,and identify important gene resources for the cultivation of cold-tolerant cucumber.
万方数据
维普
