SSR Marker Analysis and Digital Fingerprinting of Major Wine Grape Germplasm Resources in Xinjiang,China
Xinjiang boasts a rich diversity of wine grape germplasm resources,however,issues such as such as complicated genetic backgrounds and the existence of various distinct varieties are prevalent in the region.In order to effectively differentiate and optimize the utilization of the main wine grape germplasm resources cultivated in Xinjiang,110 germplasms were collected as experimental materi-als.Polymorphic SSR primers were selected for PCR amplification,followed by analysis of polymor-phism,genetic diversity and genetic distance,and construction of the digital fingerprints of germ-plasm.The results showed that 30 pairs of polymorphism-rich primers were selected from 76 pairs of SSR primers,and 391 allelic loci were amplified,with a polymorphism rate 97.164％.Among these,the primer VVIV67 had the highest numbers of bands,with a total of 22.The average observed het-erozygosity(Ho)of each locus was 0.671,the expected heterozygosity(He)was 0.790,and the Shannon's information index(Ⅰ)ranged from 1.142 to 2.627,with an average PIC value of 0.766.Genetic distance variation mainly ranged from 0.500 to 1.000,accounting for 92.399％of the total variation.The clustering analysis divided the test materials into 6 groups.Groups Ⅰ,Ⅱ and Ⅲ pre-dominantly included varieties introduced from France,the former Soviet Union,the United States,Italy,and other countries,while groups Ⅳ and Ⅴ were mostly composed of the interspecific and in-traspecific hybrids of China's Vitis amurensis Rupr.varieties,group Ⅵ represented Vitis amurensis Rupr.varieties selected by China.Principal component analysis showed that the Vitis vinifera L.and Vitis amurensis Rupr.were genetically distant,and the Vitis vinifera L.varieties were genetically close.Genetic structure analysis aggregated the wine grape core into two clusters of intraspecific or in-terspecific hybrids with Vitis vinifera L.and Vitis amurensis Rupr.as parents.The digital finger-prints accurately identified the various germplasms,with'11-22-16'and'12-10-60','Kelemuboxi'and'Cabernet Sauvignon 170','Генусаa Чибил'and'Тавриц','Zijingmenglu'and'1-5-7'potentially sharing identical names.The SSR loci of the main wine grape varieties cultivated in Xinjiang are abundant and useful,and the experimental materials exhibit rich genetic diversity,which can provide a foundation for the utilisation of wine grape germplasm resources and varietal selection and breeding.
万方数据
维普
