Resistance Detection of Botrytis cinerea to Boscalid and Biological Traits in Shanxi Province
To elucidate the resistance of Botrytis cinerea to boscalid in protected vegetable of Shanxi province,the resistance levels of 312 B.cinerea isolates to boscalid were determined by the mycelium growth rate method.The mutation sites in the SdhB gene and biological fitness,and the cross-resist-ance between boscalid and the other four fungicides(pyraclostrobin,difenoconazole,pyrimethanil and prochloraz)were investigated in both boscalid-resistant and sensitive isolates.The results showed that B.cinerea isolates exhibited resistance to boscalid(resistance frequency of 46.15％),with the resist-ance types being predominantly low levels of resistance.Resistance to boscalid of B.cinerea was dif-ferent in various regions.Among these regions,the resistance frequencies of B.cinerea isolates in Jin-nan,Lüliang,and Taiyuan regions were all greater than 50％.However,the resistance frequencies in Xinzhou and Yangquan regions were lower,with 20.00％and 10.81％,respectively.Resistance mech-anism analysis showed that all nine resistant isolates had single point mutations,including P225F,N230I,and H272R.Additionally,the mycelial growth rate,sclerotinia production ability and patho-genicity of the resistant isolates were not significantly different from those of the susceptible isolates,although the spore production decreased significantly.No cross-resistance was observed between boscalid and pyraclostrobin,difenoconazole,pyrimethanil,or prochloraz.The resistance of B.cinerea to boscalid in protected vegetables of Shanxi province was continuously increasing,with the biological fitness of resistant isolates being similar to that of susceptible isolates.Therefore,the use of boscalid should be limited during the later stages of gray mold prevention,and the four fungicides-pyra-clostrobin,difenoconazole,pyrimethanil,and prochlorazare recommended for rotational or combined use.
万方数据
维普
