摘要
为明确甘肃民勤青土湖肉苁蓉茎腐病病原菌种类,采用组织分离法,结合形态学特征与ITS分子方法对其进行分离鉴定.结果表明,从青土湖肉苁蓉茎腐病中共分离出3种不同的病原菌,室内回接和田间发病症状基本一致,均表现出茎腐病典型症状,分别命名为RCRJF-1、RCRJF-2、RCRJF-3.在PSA培养基上观察3个菌株的形态特征得知,RCRJF-1菌落圆形,淡紫色,白色气生菌丝,背面产生淡紫色色素,大型分生孢子细长,镰刀型,2～5隔,小型分生孢子长椭圆形,多无隔;RCRJF-2具有白色气生菌丝,菌落淡粉色,菌落背面中心淡紫色,但在PSA培养基上不产孢,在PDA培养基上大型分生孢子2～3分隔,小型分生孢子0～1分隔,卵圆形或肾形,厚垣孢子球形,间生或串生;RCRJF-3具有棉絮状气生菌丝,表面玫瑰红色、边缘玫瑰红色、中心部分黄棕色,菌落背面紫红色,在PDA培养基上不产孢,在PSA培养基上大型分生孢子镰刀型,多数3～5隔,小型分生孢子较少,成球棒状.进一步ITS分子鉴定得知,RCRJF-1、RCRJF-2、RCRJF-3分别与层出镰刀菌(Fusarium proliferatum)(登录号 MT560212)、尖孢镰刀菌(Fusarium oxysporum)(登录号MT530243)、锐顶镰刀菌(Fusarium acuminatum)(登录号MH341217)具有较高的相似性,均为100％.结合形态学特征,确定引起甘肃民勤青土湖肉苁蓉茎腐病的致病菌为层出镰刀菌(F.proliferatum)、尖孢镰刀菌(F.oxysporum)和锐顶镰刀菌(F.acuminatum),分离频率分别为52.38％、31.11％和16.51％,其中层出镰刀菌(F.proliferatum)为优势致病菌.
Abstract
To clarify pathogen species causing stem rot in Cistanche deserticola at Qingtu Lake of Minqin,Gansu Province,the tissue isolation method,morphological characteristics and ITS molecular methods were used for pathogen isolation and identification.The results showed that three different pathogens were isolated from stem rot in C.deserticola at Qingtu Lake,the symptoms observed both in indoor and field were essentially the same,showing typical characteristics of stem rot.These patho-gens were designated as RCRJF-1,RCRJF-2,and RCRJF-3,respectively.The ITS molecular identifica-tion showed that RCRJF-1,RCRJF-2 and RCRJF-3 exhibited 100％similarity to Fusarium prolifera-tum(accession number MT560212),Fusarium oxysporum(accession number MT530243)and Fusar-ium acuminatum(accession number MH341217),respectively.Based on morphological characteris-tics,it was determined that the pathogens causing the stem rot in C.deserticola in Qingtu Lake,Min-qin,Gansu were F.proliferatum,F.oxysporum,and F.acuminatum.The isolation frequencies were 52.38％,31.11％,and 16.51％,respectively,with F.proliferatum identified as the dominant pathogen.
维普
万方数据