Identification and Evaluation of Salt-alkali Tolerance of Faba Bean Germplasm Resources at Germination Stage and Selection of Salt-alkali Tolerant Germplasm
To evaluate the salt-alkali tolerance at germination stage of Faba Bean germplasm resources and screen materials with high salt-alkali tolerance,saline-alkali stress at the germination stage was conducted on 322 core germplasm using a mixed saline-alkali solution(4.5 g·L-1 NaCl,Na2CO3,and Na2SO4 in a ratio of 9:1:1,pH=9.2)and measured eight germination stage traits,including germination vigor,germination rate,root length,shoot length,root fresh mass,shoot fresh mass,root dry mass,and shoot dry mass.The salt-alkali tolerance of germplasm was identified and evalua-ted by membership function analysis.The results showed that mixed salt-alkali stress had an inhibito-ry effect on eight indicators.There was a significant correlation among eight indicators.The eight in-dicators were transformed into two principal components by principal component analysis,with a cu-mulative contribution rate of 68.83％.Through a combination of membership function analysis and cluster analysis,the 322 Faba Bean resources were divided into five levels,including 22 high salt-alka-li tolerant materials,64 salt-alkali tolerant materials,110 moderate salt-alkali tolerant materials,65 salt-alkali sensitive materials,and 61 salt-alkali extremely sensitive materials.In conclusion,germi-nation rate can be used as the preferred indicator for evaluating salt-alkali tolerance of faba bean at the germination stage,followed by root dry mass,shoot dry mass,and germination vigor.22 germplasm with high salt-alkali tolerance were screened out,providing superior materials for salt-alkali tolerance breeding of Faba Bean and related gene mining.
Vicia faba L.Salt-alkali stressMembership function
万方数据
维普
