Effects of Tillage and Mulching on Nitrogen Cycling Functional Microbes in the Rhizosphere Soil of Potatoes
To address the differences in cultivated land quality and potato yield caused by different till-age methods and plastic mulching in the Longdong region of Gansu,we conducted an experiment at the Lingtai Potato Field Experimental Base of the Gansu Academy of Agricultural Sciences.The study investigated the effects of tillage methods(vertical deep rotary tillage,traditional tillage)and mulc-hing(plastic mulching,no mulching)on nitrogen cycling functional microbes in the rhizosphere soil of potato fields.The results showed that compared to traditional tillage,vertical deep rotary tillage in-creased the abundance of amoA-AOA and nifHa genes by 16.8％and 46.7％,respectively,and en-hanced soil NH4+-N,soil moisture content,and the proportion of soil aggregates ≥2 mm by 5.9％,36.4％,and 60.6％,respectively.Compared to no mulching,plastic mulching increased the abun-dance of amoA-AOA,amoA-AOB,nirK,nosZ,and nifH_a genes by 64.5％,47.6％,41.6％,30.9％,and 170.5％,respectively.It increased soil NO3--N content,soil moisture content,and soil temperature by 12.6％,30.3％,and 18.9％,respectively,while reducing available phosphorus and potassium(AK)contents by 31.4％and 11.4％,respectively.Vertical deep rotary tillage with mulc-hing increased NH4-N content,soil moisture content,and soil temperature by 3.9％-7.9％,29.5％-78.1％,and 1.7％-20.9％,respectively.It also reduced(AK),available potassium con-tent,and total aggregate content by 27.8％-38.7％,0.6％-17.9％,and 0.7％-33.4％,respec-tively.Redundancy analysis indicated that all functional genes had significantly positive correlation with soil temperature,soil aggregates ≥2 mm,AK,and soil moisture content,while showing signif-icant negative correlations with soil pH and organic carbon.The amoA-AOA,amoA-AOB,and nifH_a genes were positively correlated with AK,whereas the nirK gene was negatively correlated with AK.In addition,soil temperature and available phosphorus were identified as key factors influencing nitrogen cycling functional genes.
万方数据
维普
