Analysis of Soil Microbial Diversity in Different Habitats inan Arid,Saline-alkali Area of Qinghai Province
To explore the relationship between soil microbial community diversity and soil physical and chemical properties across different habitats,samples were collected from cultivated soil,non-cul-tivated soil,desert soil,and saline-alkali soil.The physical and chemical properties of these soil sam-ples were measured.For the analysis of microbial community composition and diversity,the Illumina MisEQ platform was used for 16S amplicon sequencing.The relationship between soil flora and soil physical and chemical properties was also analyzed.The results showed that the order of microbial community diversity was cultivated soil＞saline-alkali soil＞non-cultivated soil＞desert soil.The composition and abundance of the dominant bacterial groups(phyla and genera)varied among the dif-ferent regions.The dominant bacteria in the cultivated soils were Proteobacteria,Bacteroidetes,Acti-nobacteria,and Blastomonas.The abundance of Bacillus monophyta in non-cultivated soil was lower than that in cultivated soil,whereas the abundances of Bacteroidetes and Actinobacteria were higher.The dominant bacterial phyla in desert and saline soils were Proteobacteria,Bacteroidetes,Actinobac-teria,and Firmicutes.The abundance of Firmicutes was higher in saline soil than in desert soil,where-as the opposite was true for the abundance of Actinobacteria.Sphingomonas was the most dominant genus in cultivated soils,whereas Halomonas was the most dominant genus in non-cultivated,desert,and saline soils.The correlation analysis between physical and chemical factors and soil microbial com-munity showed that electrical conductivity and water-soluble salt content were significantly positively correlated with microbial community diversity(P＜0.01),whereas organic carbon content was not significantly correlated with bacterial community diversity.These results indicate that electrical conductivi-ty and water-soluble salt content are the main factors affecting soil microbial community diversity.
Soil microbeCommunityDiversityPhysical and chemical properties
万方数据
维普
