Study on the in vitro fermentation of different types of monosaccharides by gut microbiota
The structural characteristics of carbohydrates affect their utilization efficiency by microorganisms.In order to explore the effect of their structure on gut microbiota,this article starts from the perspective of monosaccharide composition and studies the changes in gut mi-crobiota and its metabolites during in vitro culture using nine monosaccharides including glu-cose,mannose,arabinose,galactose,fucose,xylose,xylose,rhamnose,glucuronic acid,and ga-lacturonic acid as carbon sources.Mainly tested indicators such as OD600,sugar consumption,short chain fatty acids,and lactate content.After screening,the monosaccharide composition of the culture medium for mixed fermentation of seven neutral monosaccharides was ana-lyzed,and the utilization order of the seven neutral monosaccharides in the fermentation process was clarified.The diversity and abundance changes of gut microbiota were analyzed using high-throughput sequencing of 16S rRNA.It was found that different monosaccharides compete with beneficial bacteria such as lactobacilli in the process of utilization.Mannose and galactose are beneficial for the growth and reproduction of beneficial bacteria such as Lacto-bacilli in the gut.The research results will provide a theoretical basis for the study of the structure-activity relationship between polysaccharides and gut microbiota.
万方数据
维普
