Study on the antibacterial activity of lipopeptide produced by Bacillus amyloliquefaciens wxy-b3 against Alternaria mali
Alternaria mali is the common disease in apple cultivation,which seriously jeopard-ises the yield of its crops.As an antimicrobial agent mainly derived from microbial secondary metabolites,lipopeptide has the advantages of broad-spectrum inhibition,low drug resist-ance,safety and low toxicity.This study showed that lipopeptides produced by Bacillus amyloliquef aciens wxy-b3 were able to inhibit the growth of A.mali,and the minimum in-hibitory concentration of A.mali was 16 mg/mL,and caused hyperpolarisation of cell mem-brane potential,depolarisation of mitochondrial membrane potential,leakage of macromole-cule proteins and nucleic acids,as well as cumulative increase of intracellular reactive oxygen species and malondialdehyde content of A.mali,respectively 0.33 and 5.44 times,and the wrinkling of the cell morphology and the breakage of the cell membrane in the MIC-treated group were further observed by scanning electron microscopy.The above results indicated that lipopeptides could achieve the antibacterial effect by disrupting the cell membrane sys-tem,leading to changes in cell morphology,cell membrane rupture,and altering cell mem-brane permeability.This study will provide a theoretical basis for the use of lipopeptides as antimicrobial agents for the inhibition of A.mali.
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