Study on co-culture of Escherichia coli and Corynebacterium glutamicum for the biosynthesis of eriodictyol
Micro bial co-cultures are widely used in biomanufacturing and play important roles in the microbial biosynthesis of natural products.The plant natural product eriodictyol can be potentially applied in pharmaceutical and food industries due to its antimicrobial,anti-oxida-tive activities and other medical properties.To achieve de novo biosynthesis of eriodictyol from the cheap carbon source glucose,the co-culture strategy was adopted utilizing Esche-richia coli strain BAK101 and Corynebacterium glutamicum strain NE1,in which BAK101 could convert glucose into L-tyrosine,whereas NE1 was able to metabolize L-tyrosine into eriodictyol.Via system optimization combined with fed-batch fermentation and segmented co-cultivation,de novo biosynthesis of eriodictyol was accomplished with a titer of 4.72 mg/L in shake flasks from 2％glucose in 100 h of fermentation.This study sets an example for the bi-osynthesis of flavonoid compounds using microbial co-cultures,and provides guidance to the de novo microbial biosynthesis of complicated natural products.
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