The Effect of Regnase-1 Knockout on the Cytokine Secretion from Cord Blood Derived CAR-T Cells
As CAR-T(chimeric antigen receptor T)cells are continually modified to enhance their anti-tu-mor efficacy,attention must also be paid to the cytokine profiles secreted by activated CAR-T cells.These cytokines are important factors in causing the CRS(cytokine release syndrome),which is closely related to the clinical safety of CAR-T.Regnase-1 is a ribonuclease that negatively regulates the immune response.Using CRISPR/Cas9 gene editing technology,Regnase-1-deficient CAR-T cells derived from CB(cord blood)were successfully prepared.It was found that although the specific killing ability of Regnase-1-CAR-T cells was significantly stronger than that of CAR-T cells,the secretion of pro-inflammatory cytokines by Regnase-1-CAR-T cells was also significantly in-creased,indicating that Regnase-1-CAR-T cells were potentially risky in terms of clinical safety.
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