miR-106b-5p靶向调控SIRT7/SMAD4信号通路对口腔鳞状细胞癌侵袭和迁移的影响机制探究
Mechanism of miR-106b-5p on Invasion and Migration of Oral Squamous Cell Carcinoma through Targeted Regulation of SIRT7/SMAD4 Signaling Pathway
肖旭 1李泽濛 2刘佩 2李鹏程1
作者信息
- 1. 海南医学院第一附属医院口腔颌面外科,海口 570100
- 2. 海南医学院第二附属医院口腔科,海口 570311
- 折叠
摘要
该文探讨了 miR-106b-5p对口腔鳞状细胞癌(OSCC)细胞迁移和侵袭的影响及其机制.采用qRT-PCR检测OSCC组织与细胞系中miR-106b-5p表达情况,将SCC15、OECM1细胞分为Con-trol 组、miR-NC组、miR-106b-5p mimics组、anti-miR-NC组、anti-miR-106b-5p、anti-miR-106b-5p+si-NC组、anti-miR-106b-5p+si-SIRT7组,分别检测各组细胞增殖、迁移及侵袭能力,Western blot检测E-cadherin、N-cadherin、MMP-9、SIRT7、SMAD4蛋白表达情况,双荧光素酶报告基因实验与R1P实验验证miR-106b-5p与SIRT7的靶向关系.结果显示,miR-106b-5p在OSCC组织与细胞中表达水平升高(P＜0.05),过表达miR-106b-5p可显著促进OSCC细胞增殖、迁移、侵袭及EMT,抑制miR-106b-5p表达可显著抑制OSCC细胞增殖、迁移、侵袭及EMT(P＜0.05);双荧光素酶报告基因实验与RIP实验证实,miR-106b-5p与SIRT7存在靶向关系(P＜0.05);抑制SIRT7表达可逆转抑制miR-106b-5p表达对OSCC细胞增殖、迁移、侵袭及EMT的抑制作用(P＜0.05).总之,miR-106b-5p在OSCC组织与细胞中上调表达,抑制miR-106b-5p表达可通过调节S1RT7/SMAD4信号通路,抑制OSCC细胞增殖、迁移、侵袭及EMT.
Abstract
This study aimed to investigate the effects and mechanism of miR-106b-5p on migration and invasion of OSCC(oral squamous cell carcinoma)cells.The expression of miR-106b-5p in OSCC tissues and cell lines was detected by qRT-PCR.SCC15 and OECM1 cells were divided into Control group,miR-NC group,miR-106b-5p mimics group,anti-miR-NC group,anti-miR-106b-5p group,anti-miR-106b-5p+si-NC group,and anti-miR-106b-5p+si-SIRT7 group,respectively.Cell proliferation,migration and invasion were detected in each group;Western blot was used to detect the protein expression of E-cadherin,N-cadherin,MMP-9,SIRT7 and SMAD4;double luciferase reporter gene assay and RIP assay verified the targeting relationship between miR-106b-5p and SIRT7.The results showed that the expression levels of miR-106b-5p in OSCC tissues and cells were increased(P＜0.05).Overexpression of miR-106b-5p could significantly promote the proliferation,migration,invasion and EMT of OSCC cells,and inhibition of miR-106b-5p expression could significantly inhibit the proliferation,migra-tion,invasion and EMT of OSCC cells(P＜0.05).Double luciferase reporter gene experiment and RIP experiment confirmed that there was a targeting relationship between miR-106b-5p and SIRT7(P＜0.05).Inhibition of SIRT7 expression could reverse the inhibitory effects of miR-106b-5p expression on the proliferation,migration,invasion and EMT of OSCC cells(P＜0.05).In conclusion,the expression of miR-106b-5p is up-regulated in OSCC tis-sues and cells.Inhibition of miR-106b-5p expression can inhibit the proliferation,migration,invasion and EMT of OSCC cells by regulating SIRT7/SMAD4 signal pathway.
关键词
微小RNA-106b-5p/口腔鳞状细胞癌/迁移/侵袭/SIRT7/SMAD4Key words
miR-106b-5p/oral squamous cell carcinoma/migration/invasion/SIRT7/SMAD4引用本文复制引用
基金项目
海南省卫生健康行业科研项目(21A200239)
出版年
2024
NETL
NSTL
万方数据