Mechanism of miR-106b-5p on Invasion and Migration of Oral Squamous Cell Carcinoma through Targeted Regulation of SIRT7/SMAD4 Signaling Pathway
This study aimed to investigate the effects and mechanism of miR-106b-5p on migration and invasion of OSCC(oral squamous cell carcinoma)cells.The expression of miR-106b-5p in OSCC tissues and cell lines was detected by qRT-PCR.SCC15 and OECM1 cells were divided into Control group,miR-NC group,miR-106b-5p mimics group,anti-miR-NC group,anti-miR-106b-5p group,anti-miR-106b-5p+si-NC group,and anti-miR-106b-5p+si-SIRT7 group,respectively.Cell proliferation,migration and invasion were detected in each group;Western blot was used to detect the protein expression of E-cadherin,N-cadherin,MMP-9,SIRT7 and SMAD4;double luciferase reporter gene assay and RIP assay verified the targeting relationship between miR-106b-5p and SIRT7.The results showed that the expression levels of miR-106b-5p in OSCC tissues and cells were increased(P＜0.05).Overexpression of miR-106b-5p could significantly promote the proliferation,migration,invasion and EMT of OSCC cells,and inhibition of miR-106b-5p expression could significantly inhibit the proliferation,migra-tion,invasion and EMT of OSCC cells(P＜0.05).Double luciferase reporter gene experiment and RIP experiment confirmed that there was a targeting relationship between miR-106b-5p and SIRT7(P＜0.05).Inhibition of SIRT7 expression could reverse the inhibitory effects of miR-106b-5p expression on the proliferation,migration,invasion and EMT of OSCC cells(P＜0.05).In conclusion,the expression of miR-106b-5p is up-regulated in OSCC tis-sues and cells.Inhibition of miR-106b-5p expression can inhibit the proliferation,migration,invasion and EMT of OSCC cells by regulating SIRT7/SMAD4 signal pathway.
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