Development and Research of Nucleic Acid Detection Technology based on Class 2 Type Ⅴ CRISPR-Cas System
Nucleic acid detection technology is widely used in pathogen detection due to its high sensitiv-ity and strong specificity,and plays an important role in public health management and clinical treatment decision making.Moreover,detection technology based on Class 2 type Ⅴ CRISPR-Cas system possesses attributes such as simplicity,rapidity,and exhibits promising potential for further applications.This review provides a concise over-view of the composition,structure,function,and mechanism of the Class 2 type Ⅴ CRISPR-Cas system.It further highlights the various nucleic acid detection platforms developed using different Ⅴ-type Cas proteins and discusses their corresponding application scenarios,concluding with a brief outlook on their future prospects.
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