基于CRISPR 2类Ⅴ型系统的检测技术开发与研究进展
Development and Research of Nucleic Acid Detection Technology based on Class 2 Type Ⅴ CRISPR-Cas System
李宏宇 1黄诗晴 1郑巍薇 1欧阳松应1
作者信息
- 1. 福建师范大学生命科学学院,福州 350117
- 折叠
摘要
核酸检测技术以其灵敏度高、特异性强的特点,广泛应用于病原体检测中,在公共卫生管理和临床治疗决策等场景中发挥重要作用.基于CRISPR 2类Ⅴ型系统的核酸检测技术,同时还具备检测方式简单、检测时间短的特点,具有良好的发展前景.该文简要介绍CRISPR 2类Ⅴ型系统的组成、结构、功能与作用机制,总结已开发的基于不同Ⅴ型Cas蛋白的众多检测平台以及这些检测平台的应用场景,并对其未来发展进行简要述评.
Abstract
Nucleic acid detection technology is widely used in pathogen detection due to its high sensitiv-ity and strong specificity,and plays an important role in public health management and clinical treatment decision making.Moreover,detection technology based on Class 2 type Ⅴ CRISPR-Cas system possesses attributes such as simplicity,rapidity,and exhibits promising potential for further applications.This review provides a concise over-view of the composition,structure,function,and mechanism of the Class 2 type Ⅴ CRISPR-Cas system.It further highlights the various nucleic acid detection platforms developed using different Ⅴ-type Cas proteins and discusses their corresponding application scenarios,concluding with a brief outlook on their future prospects.
关键词
CRISPR-Cas系统/核酸检测/病原微生物检测Key words
CRISPR-Cas system/nucleic acid detection/pathogenic microorganism detection引用本文复制引用
基金项目
国家自然科学基金(31770948)
福建省自然科学基金(2022J01638)
出版年
2024
NETL
NSTL
万方数据