Resveratrol Improves Cognitive Impairments Caused by Adolescent Intermittent Ethanol Exposure in Adult Rats
This study aimed to investigate the effects of RSV(resveratrol)on cognitive function in rats exposed to AIE(adolescent intermittent ethanol)and its potential mechanisms.AIE-exposed rat models were es-tablished by intermittent gavage of 25％ethanol and the RSV or the Veh(vehicle)was administered intragastri-cally.The results of the open field test showed that compared with the Con+Veh group,the number of center zone entries(P＜0.01)and the distance in center(P＜0.001)of the Eth+Veh group were significantly reduced.After RSV treatment,compared with the Eth+Veh group,the number of center zone entries(P＜0.01)and the distance in center(P＜0.05)of the Eth+RSV group were significantly increased.The results of the elevated plus maze test showed that compared with the Con+Veh group,the open arm duration and the open arm entries of rats in the Eth+Veh group were significantly reduced(P＜0.01).Compared with the Eth+Veh group,the open arm entries of rats in the Eth+RSV group were significantly increased(P＜0.05),but the open arm entries were no statistical dif-ference(P＞0.05).The results of the Morris water maze test showed that compared with the Con+Veh group,the latency to target of the Eth+Veh group was significantly increased(P＜0.01)and the target crossings were signifi-cantly decreased(P＜0.05).Compared with the Eth+Veh group,the latency to target and the target crossings of the Eth+RSV group were significantly decreased(P＜0.05).The results of Nissl staining showed that compared with the Con+Veh group,the number of neurons in the mPFC(medial prefrontal cortex)in the Eth+Veh group was signifi-cantly decreased(P＜0.001).Compared with the Eth+Veh group,the number of neurons in the Eth+RSV group was significantly increased(P＜0.01).Western blot results showed that compared with the Con+Veh group,the protein expression levels of SIRT1(P＜0.05),BDNF(P＜0.05),and PSD95(P＜0.05)in mPFC of rats in the Eth+Veh group were significantly decreased.Compared with the Eth+Veh group,the protein expression levels of SIRT1(P＜0.01),BDNF(P＜0.05),and PSD95(P＜0.05)in the mPFC of rats in the Eth+RSV group were significantly increased.These results suggest that RSV can improve the cognitive impairment induced by AIE in rats and increase the num-ber of neuronal in mPFC,which may be related to the increased expression of SIRT1,BDNF,and PSD95 in mPFC.
resveratroladolescent intermittent ethanolcognitive functionsilent information regulator 1brain-derived neurotrophic factorpostsynaptic density protein 95
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