首页|白藜芦醇改善青春期酒精暴露导致的成年期大鼠认知功能损害

白藜芦醇改善青春期酒精暴露导致的成年期大鼠认知功能损害

Resveratrol Improves Cognitive Impairments Caused by Adolescent Intermittent Ethanol Exposure in Adult Rats

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该文旨在研究白藜芦醇(RSV)对青春期间歇性酒精(AIE)暴露大鼠认知功能的影响及潜在的机制.通过25％酒精灌胃建立AIE暴露大鼠模型,并使用RSV进行干预.旷场实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠进入中心区域的次数(P＜0.01)、在中心区域活动距离减少(P＜0.001).RSV治疗后,Eth+RSV组大鼠较Eth+Veh组进入中心区域的次数(P＜0.01)、在中心区域活动距离增加(P＜0.05).高架十字迷宫实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠开放臂停留时间(P＜0.01)、进入开放臂次数显著减少(P＜0.01).RSV治疗后,Eth+RSV组大鼠较Eth+Veh组进入开放臂次数显著增加(P＜0.05),在开放臂停留时间有增多趋势,但无统计学差异(P＞0.05).Morris水迷宫实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠测试期逃避潜伏期增加(P＜0.01)、穿越目标平台次数明显减少(P＜0.05).而RSV治疗后,Eth+RSV组大鼠较Eth+Veh组测试期逃避潜伏期降低(P＜0.05)、穿越目标平台次数明显增多(P＜0.05).尼氏染色结果显示,与Con+Veh组相比,Eth+Veh组大鼠内侧前额叶皮层(mPFC)神经元数量显著减少(P＜0.001).与Eth+Veh组相比,Eth+RSV组大鼠mPFC神经元尼氏体数量显著增多(P＜0.01).Western blot结果显示,与Con+Veh组相比,Eth+Veh组大鼠mPFC 内 SIRT1(P＜0.05)、BDNF(P＜0.05)及PSD95(P＜0.05)蛋白表达水平显著降低.与Eth+Veh组相比,Eth+RSV组大鼠mPFC内SIRT1(P＜0.01)、BDNF(P＜0.05)及PSD95(P＜0.05)蛋白表达水平显著增高.该研究结果表明,RSV能够改善AIE导致的大鼠认知功能损害,并增加mPFC内神经元尼氏体数量,这种改善作用可能与mPFC中SIRT1、BDNF及PSD95的表达水平增加相关.
This study aimed to investigate the effects of RSV(resveratrol)on cognitive function in rats exposed to AIE(adolescent intermittent ethanol)and its potential mechanisms.AIE-exposed rat models were es-tablished by intermittent gavage of 25％ethanol and the RSV or the Veh(vehicle)was administered intragastri-cally.The results of the open field test showed that compared with the Con+Veh group,the number of center zone entries(P＜0.01)and the distance in center(P＜0.001)of the Eth+Veh group were significantly reduced.After RSV treatment,compared with the Eth+Veh group,the number of center zone entries(P＜0.01)and the distance in center(P＜0.05)of the Eth+RSV group were significantly increased.The results of the elevated plus maze test showed that compared with the Con+Veh group,the open arm duration and the open arm entries of rats in the Eth+Veh group were significantly reduced(P＜0.01).Compared with the Eth+Veh group,the open arm entries of rats in the Eth+RSV group were significantly increased(P＜0.05),but the open arm entries were no statistical dif-ference(P＞0.05).The results of the Morris water maze test showed that compared with the Con+Veh group,the latency to target of the Eth+Veh group was significantly increased(P＜0.01)and the target crossings were signifi-cantly decreased(P＜0.05).Compared with the Eth+Veh group,the latency to target and the target crossings of the Eth+RSV group were significantly decreased(P＜0.05).The results of Nissl staining showed that compared with the Con+Veh group,the number of neurons in the mPFC(medial prefrontal cortex)in the Eth+Veh group was signifi-cantly decreased(P＜0.001).Compared with the Eth+Veh group,the number of neurons in the Eth+RSV group was significantly increased(P＜0.01).Western blot results showed that compared with the Con+Veh group,the protein expression levels of SIRT1(P＜0.05),BDNF(P＜0.05),and PSD95(P＜0.05)in mPFC of rats in the Eth+Veh group were significantly decreased.Compared with the Eth+Veh group,the protein expression levels of SIRT1(P＜0.01),BDNF(P＜0.05),and PSD95(P＜0.05)in the mPFC of rats in the Eth+RSV group were significantly increased.These results suggest that RSV can improve the cognitive impairment induced by AIE in rats and increase the num-ber of neuronal in mPFC,which may be related to the increased expression of SIRT1,BDNF,and PSD95 in mPFC.

resveratroladolescent intermittent ethanolcognitive functionsilent information regulator 1brain-derived neurotrophic factorpostsynaptic density protein 95

贾文歌、刘媛、王心雨、李昊隆、曾令宇、杨一帆、李晨晨、陈红云、赵斌、魏来

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新乡医学院法医学院,新乡市法医毒理重点实验室,新乡 453003

白藜芦醇 青春期间歇性酒精暴露 认知功能 沉默信息调节因子1 脑源性神经营养因子 突触后密度蛋白95

国家自然科学基金新乡医学院研究生科研创新支持计划河南省高等学校大学生创新创业训练计划

U2004111YJSCX202289Y202210472021

2024

10.11844/cjcb.2024.03.0007

中国细胞生物学学报
中国科学院上海生命科学研究院,生物化学与细胞生物学研究所,中国细胞生物学学会

中国细胞生物学学报

CSTPCD
影响因子：0.554
ISSN：1674-7666
年,卷(期)：2024.46(3)
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