This study focused on the effects of Lido(lidocaine)on the biological behavior of CRC(colorectal cancer)cells by regulating the RhoA(Ras homologous gene family member A)/ROCK(Rho associated coiled-coil forming protein kinase)axis.In this study,human colorectal cancer cells LS513 were treated with 0～1 250 μmol/L lidocaine,and cell viability was detected by CCK-8 method to screen suitable drug concentration.The cells were grouped into Control group,lidocaine low concentration group(Lido-L group,500 μmol/L Lido),lidocaine medium concentration group(Lido-M group,750 μmol/L Lido),lidocaine high concentration group(Lido-H group,1 000 μmol/L Lido)and lidocaine high concentration+ROCK signal pathway activator LPA group(Lido-H+LPA group,1 000 μmol/L Lido+10 μmol/L LPA group).Edu was applied to detect cell proliferation;scratch heal-ing test and transwell chamber test were applied to detect cell migration and invasion ability,respectively;flow cy-tometry was applied to detect cell apoptosis;Western blot was applied to detect the expression of PCNA,Bax,Bcl-2,RhoA,ROCK 1,E-cadherin,and N-cadherin proteins.In this study,compared with 0 μmol/L Lido,the activity of LS513 cells treated with 500 μmol/L,750 μmol/L,1 000 μmol/L and 1 250 μmol/L Lido obviously decreased(P＜0.05),lidocaine of 500 μmol/L,750 μmol/L and 1 000 μmol/L was selected for subsequent experiments.Compared with the Control group,the Edu positive rate,scratch healing rate,cell invasion number,and PCNA,N-cadherin,Bcl-2,RhoA,and ROCK 1 protein expression of LS513 cells in the Lido-L group,Lido-M group,and Lido-H group decreased sequentially(P＜0.05),the apoptosis rate,E-cadherin and Bax protein expression increased sequentially(P＜0.05);compared with the Lido-H group,the Edu positive rate,scratch healing rate,cell invasion number,and PCNA,N-cadherin,Bcl-2,RhoA,and ROCK 1 protein expression of LS513 cells in the Lido-H+LPA group obviously increased(P＜0.05),the apoptosis rate,E-cadherin,and Bax protein expression were obviously reduced(P＜0.05).Lidocaine may inhibit the malignant biological behavior of colorectal cancer cells by inhibiting RhoA/ROCK signaling pathway.
lidocaineRas homolog gene family member A/Rho associated coiled-coil forming protein ki-nase signal pathwaycolorectal cancermalignant biological behavior
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