Berberine Regulates the Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells through PI3K/AKT Signaling Pathway
This study aims to investigate the effect and potential mechanism of berberine on the pro-liferation and osteogenic differentiation of hPDLSCs(human periodontal ligament stem cells).hPDLSCs were cultured in vitro,and were divided into blank Con(control)group,LY(PI3K/AKT signaling pathway inhibi-tor LY294002)group,Ber(berberine)group and Ber+LY(berberine+PI3K/AKT signaling pathway inhibitor LY294002)group.CCK-8(cell counting kit-8)method was used to detect the proliferation activity of hPDLSCs.Flow cytometry was used to detect cell cycle distribution.Enzyme-linked immunosorbent assay was used to de-tect ALP(alkaline phosphatase)activity.qRT-PCR(real-time fluorescent quantitative PCR)was used to analysis the expression of PCNA(proliferating cell nuclear antigen),Cyclin D1,OCN(osteocalcin),POSTN(periostin)and OPN(osteopontin).Western blot was used to detect the expression of PCNA,Cyclin D1,OCN,POSTN,OPN and PI3K/AKT signaling pathway-related proteins.Compared with the Con group,the proliferation activ-ity of hPDLSCs in the Ber group,ALP activity,S phase and G2/M phase cell ratio,PCNA,Cyclin D1,OCN,POSTN,OPN,p-PI3K and p-AKT expression levels were significant increased,the proportion of cells in G0/G1 phase was significantly reduced(P＜0.05),while the above indicators in the LY group showed opposite changes(P＜0.05).Compared with the Ber group,the proliferation activity of hPDLSCs in the Ber+LY group,the activity of ALP,the ratio of cells in S phase and G2/M phase,the expression levels of PCNA,Cyclin D1,OCN,POSTN,OPN,p-PI3K and p-AKT were significantly reduced,and the proportion of cells in G0/G1 phase was significantly increased(P＜0.05).Berberine can promote the proliferation of hPDLSCs and induce the osteogenic differentia-tion of hPDLSCs by activating the PI3K/AKT signaling pathway.
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