Impacts of lncRNA CBR3-AS1 on Proliferation,Apoptosis,and Invasion of Nasopharyngeal Carcinoma Cells by Regulating the miR-145-5p/FSCN1 Axis
This study aims to investigate the impacts of lncRNA CBR3-AS1(long non-coding RNA CBR3-AS1)on the proliferation,apoptosis and invasion of NPC(nasopharyngeal carcinoma)cells by targeting the miR-145-5p(microRNA-145-5p)/FSCN1(fascin 1)axis.The qRT-PCR method was applied to analyze the expression levels of lncRNA CBR3-AS1 and miR-145-5p in nasopharyngeal carcinoma tissues.Nasopharyngeal cancer cell CNE-1 was divided into si-NC group,si-CBR3-AS1 group,si-CBR3-AS1+anti-miR-NC group,si-CBR3-AS1+anti-miR-145-5p group,miR-NC group,miR-145-5p mimics group,miR-145-5p mimics+pcDNA group,and miR-145-5p mimics+FSCN1 group.Double luciferase experiment was applied to detect the targeting relationship between lncRNA CBR3-AS1 and miR-145-5p,and between FSCN1 and miR-145-5p;MTT method was applied to detect cell proliferation;Annexin V-FITC/PI method was applied to detect cell apoptosis;Transwell experiment was applied to detect cell invasion ability;Western blot was applied to detect the protein expression changes of P21(cell cycle negative regulator),Bcl-2(B cell lymphoblastoma-2),Bax(Bcl-2 associated X protein),MMP-2(ma-trix metalloproteinase 2),and MMP-9(matrix metalloproteinase 9).Mouse transplantation tumor experiment was applied to explore the effect of LncRNA CBR3-AS1 on the growth of nasopharyngeal carcinoma transplantation tumors and miR-145-5p/FSCN1.In nasopharyngeal carcinoma tissue,the expression of lncRNA CBR3-AS1 was up-regulated,while the expression of miR-145-5p was down-regulated(P＜0.05).Interference with lncRNA CBR3-AS1 or up-regulation of miR-145-5p expression was able to inhibit the proliferation and invasion of nasopharyngeal carcinoma cells,and promote the apoptosis of nasopharyngeal carcinoma cells(P＜0.05).Down-regulation of miR-145-5p expression or up-regulation of FSCN1 expression was able to reverse the inhibitory effects of interfering with lncRNA CBR3-AS1 expression or up-regulation of miR-145-5p expression on the proliferation and invasion of nasopharyngeal carcinoma cells(P＜0.05).The mouse transplantation tumor experiment showed that down-regu-lating the expression of lncRNA CBR3-AS1 was able to up-regulate the expression of miR-145-5p,thereby down-regulating the expression of FSCN1 and inhibiting the growth of nasopharyngeal carcinoma transplantation tumors(P＜0.05).lncRNA CBR3-AS1 is up-regulated in nasopharyngeal carcinoma tissue,down-regulating the expression of lncRNA CBR3-AS1 can inhibit the proliferation and invasion of nasopharyngeal carcinoma cells,and promote cell apoptosis by regulating the miR-145-5p/FSCN1 axis.
long non-coding RNA CBR3-AS1microRNA-145-5pfascin 1nasopharyngeal carcinomaproliferationapoptosisinvasion
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