HucMSC-Ex Regulates Autophagy to Enhance the Function of Dermal Fibroblasts in a High-Glucose Environment
The objective of this study was to investigate the impact of hucMSC-Ex(human umbilical cord mesenchymal stem cell-derived exosome)on DFs(dermal fibroblasts)in a high-glucose damage model and to ex-plore the role of autophagy in the repair of DFU(diabetic foot ulcer).A comprehensive identification of hucMSC and hucMSC-Ex was conducted using various methods,including morphological analysis,particle size measure-ment,and surface marker characterization.Additionally,surface markers of primary DFs were identified through Western blot and immunofluorescence analyses.The assessment of apoptosis,inflammatory factor expression,ROS(reactive oxygen species)production,and mitochondrial function in DFs under high-glucose damage conditions separately were performed using flow cytometry,qRT-PCR,ROS detection,and measurement of mitochondrial membrane potential.To further investigate the role of autophagy in DFU repair,the expression of LC3BⅡ/I in DFU patient tissues was examined through immunohistochemistry and Western blot techniques.Differences in the expression of autophagy-related proteins were analyzed by extracting tissue proteins.Moreover,hucMSC-Ex was administered to DFs after treatment with autophagy inducers and inhibitors to observe their proliferation and migra-tion capabilities.The research results suggest that hucMSC-Ex may enhance the function of DFs under high glucose damage by modulating the autophagic response,thereby promoting the repair of DFU.
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