Effects of Nicardipine on Proliferation,Apoptosis and Chemoresistance of Bladder Cancer Cells by Regulating RhoA/ROCK Signaling Pathway
The aim of this study was to investigate the effect of nicardipine on the proliferation,apoptosis and chemoresistance of bladder cancer cells by regulating RhoA/ROCK signaling pathway.T24 cells of blad-der cancer were separated into control group,L-nicardipine group,M-nicardipine group,H-nicardipine group,H-nicardipine+LPA group,DMSO group,cisplatin group,H-nicardipine+cisplatin group,H-nicardipine+cisplatin group,and H-nicardipine+cisplatin+LPA group.The proliferation activity,apoptosis,migration,invasion ability,and RhoA/ROCK signaling pathway protein expression of T24 cells were detected using CCK-8 assay kit,flow cytometry,scratch assay,Transwell method,and Western blot,respectively.Compared with the control group,the D value,scratch healing rate,number of invasive cells,and the RhoA and ROCK protein expression levels in the L-nicardipine group,M-nicardipine group,and H-nicardipine group were obviously reduced(P＜0.05),while the apoptosis rate of cells was obviously increased(P＜0.05).Compared with the H-nicardipine group,the D value,scratch healing rate,number of invasive cells,and the RhoA and ROCK expression levels in H-nicardipine+LPA group were obviously increased(P＜0.05),while the apoptosis rate of cells was obviously decreased(P＜0.05).Compared with the DMSO group,the D value,scratch healing rate,and number of invasive cells in the cisplatin group were decreased,while the apoptosis rate was increased(P＜0.05).Compared with the cisplatin group,the D value,scratch healing rate,and number of invasive cells in the H-nicardipine group and the H-nicardipine+cisplatin group were decreased,while the apoptosis rate was increased(P＜0.05).Compared with the H-nicardipine+cisplatin group,the H-nicardipine+cisplatin+LPA group showed an increase in D value,scratch healing rate,and number of invasive cells,and a decrease in apoptosis rate(P＜0.05).Nicardipine can inhibit the proliferation and chemoresis-tance of bladder cancer cells,and promote cell apoptosis.The mechanism may be related to the inhibition of RhoA/ROCK signaling pathway.
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