首页|碱韭总黄酮通过调控细胞程序性死亡发挥抗肿瘤作用的机制研究

碱韭总黄酮通过调控细胞程序性死亡发挥抗肿瘤作用的机制研究

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黄酮类化合物对多种肿瘤细胞具有抑制作用,但碱韭总黄酮是否具有抗肿瘤的活性尚不清楚.该研究通过细胞计数、克隆形成、划痕、侵袭、流式细胞术、实时定量PCR和免疫印迹等实验,发现碱韭总黄酮对HeLa、LLC和Hep G2三种肿瘤细胞有不同程度的抑制作用,IC50分别为(90.73±2.20)、(78.27±3.24)和(333.20±2.15)μg/mL;且能有效减弱三种细胞的增殖能力、克隆形成能力、迁移能力和侵袭能力;同时促进HeLa细胞的凋亡和LLC细胞的坏死性凋亡;分子机制上发现,经碱韭总黄酮处理后,HeLa细胞中促凋亡蛋白BAX的表达水平和BAX/BCL2值显著上调,而LLC细胞中坏死性凋亡相关蛋白RIPK3表达水平显著上调;LLC细胞成瘤实验发现碱韭总黄酮在体内也具有抑制肿瘤的活性,并且可通过促进坏死性凋亡而发挥作用.综上,该研究揭示了碱韭总黄酮在体内和体外的抗肿瘤活性,并且其对不同肿瘤细胞的抑制效果和作用机制不同,对HeLa细胞通过促进凋亡发挥抑制作用,而对LLC细胞通过促进坏死性凋亡发挥抑制作用.
The Mechanism of Total Flavonoids from Allium polyrhizum Exerting Anti-Tumor Effects by Regulating Programmed Cell Death
Flavonoids have inhibitory effects on various tumor cells,but it's not clear whether the total flavonoids of Allium polyrhizum have anti-tumor activity.In this study,the total flavonoids from Allium polyrhizum were found to inhibit three kinds of tumor cells,HeLa,LLC and Hep G2,which confirmed by cell counting,clone formation,scratch,invasion,flow cytometry,real-time quantitative PCR and immunoblotting,with IC5o values of(90.73±2.20),(78.27±3.24)and(333.20±2.15)μg/mL,respectively;and it could effectively inhibit the proliferative ability,clonogenic ability,migratory ability,and invasive ability of three types of cells;simultaneously promoting apoptosis of HeLa cells and necroptosis of LLC cells;molecular mechanisms revealed that the expression of pro-apoptotic protein BAX and BAX/BCL2 values were significantly up-regulated in HeLa cells,while the expression of necrotic apoptosis-associated protein RIPK3 was significantly up-regulated in LLC cells after treatment with total flavonoids from Allium polyrhizum;the LLC cell tumorigenesis experiment found that the total flavonoids from Allium polyrhizum also had anti-tumor activity in vivo,and exerted inhibitory effects by promoting necroptosis pathways.In summary,this study revealed that the total flavonoids from Allium polyrhizum had anti-tumor activity both in vivo and in vitro,and the inhibitory effects and mechanisms on different tumor cells were inconsistent.It ex-erted inhibitory effects on HeLa cells and LLC cells by promoting apoptosis and necroptosis,respectively.

Allium polyrhizumtotal flavonoidsanti-tumorapoptosisnecroptosis

赵彩权、赵雅茹、赵鹏、白力格

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包头师范学院,生态环境学院,包头 014030

碱韭 总黄酮 抗肿瘤 细胞凋亡 坏死性凋亡

国家自然科学基金内蒙古自治区自然科学基金项目内蒙古自治区自然科学基金项目国家级大学生创新训练计划包头师范学院高层次人才引进科研启动基金包头师范学院高层次人才引进科研启动基金

323601482021BS080042021BS08017202310131004BTTCRCQD2020-004BTTCRCQD2020-005

2024

中国细胞生物学学报
中国科学院上海生命科学研究院,生物化学与细胞生物学研究所,中国细胞生物学学会

中国细胞生物学学报

CSTPCD
影响因子:0.554
ISSN:1674-7666
年,卷(期):2024.46(7)
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