Effects of LncRNA ASB16-AS1 on the Proliferation,Migration,and Invasion of Oral Squamous Cell Carcinoma Cells by Regulating the miR-185-5p/TRAM2 Axis
This study aims to investigate the effects of LncRNA ASB16-AS 1(long non-coding RNA ASB16-AS1)on the proliferation,migration,and invasion of oral squamous cell carcinoma cells by targeting the miR-185-5p/TRAM2 axis.Human oral squamous cell carcinoma cells SCC-9 were cultured in vitro and grouped into control group,sh-NC group,sh-ASB16-AS1 group,sh-ASB16-AS1+inhibitor NC group,and sh-ASB16-ASl+miR-185-5p inhibitor group.qRT-PCR method was applied to detect the expression of LncRNA ASB16-AS1,miR-185-5p,and TRAM2 mRNA of cells in each group.SCC-9 cell proliferation was detected using CCK-8 assay kit.SCC-9 cell apoptosis was detected by flow cytometry.SCC-9 cell migration was detected using scratch assay,and SCC-9 cell invasion was detected using Transwell method.Western blot was applied to detect the expression of TRAM2 protein.Dual luciferase reporter assay was applied to verify the targeting relationship between LncRNA ASB16-AS1 and miR-185-5p,and miR-185-5p and TRAM2.Compared with the control group and sh-NC group,the expression levels of LncRNA ASB16-AS1,TRAM2 mRNA,and TRAM2 protein,as well as D value,scratch healing rate,and number of invasive cells were reduced in the sh-ASB16-AS1 group,while the level of miR-185-5p and cell apoptosis rate were increased(P＜0.05).Compared with the sh-ASB16-AS1+inhibitor NC group,the miR-185-5p level and cell apoptosis rate in the sh-ASB16-AS1+miR-185-5p inhibitor group were reduced,while the ex-pression levels of TRAM2 mRNA and TRAM2 protein,D values,scratch healing rate,and number of invasive cells were elevated(P＜0.05),however,there were no significant changes in the levels of LncRNA ASB16-AS1(P＞0.05).Biological information websites predicted that miR-185-5p had targeted binding sites with LncRNA ASB16-AS1 and TRAM2,and dual luciferase reporter gene experiments confirmed the existence of targeted relationships be-tween LncRNA ASB16-AS1 and miR-185-5p,and miR-185-5p and TRAM2(P＜0.05).The expression of LncRNA ASB16-AS1 is upregulated in oral squamous cell carcinoma cells.Inhibiting the expression of LncRNA ASB16-AS1 can target and upregulate the expression of miR-185-5p,inhibit the expression of TRAM2,promote apoptosis of oral squamous cell carcinoma cells,and inhibit their proliferation,migration,and invasion.
long non-coding RNA ASB16-AS1microRNA-185-5p/translocation associated membrane protein 2oral squamous cell carcinomaproliferationmigrationinvasion
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