DNA双链断裂高通量测序技术的研究进展
High Throughput Sequencing Technologies for DNA Double-Strand Break
吴树恒 1梁羽 1吴薇1
作者信息
- 1. 多细胞体系结构与功能重点实验室,中国科学院分子细胞科学卓越创新中心/生物化学与细胞生物学研究所,上海 200031
- 折叠
摘要
DNA双链断裂(DNA double-strand break,DSB)是最严重的DNA损伤类型,研究其产生及修复机制对理解疾病发生、发展具有非常积极的意义.测序技术的发展推动了多种DSB高通量测序技术的诞生,使得在全基因组范围内检测DSB"热点"及其修复加工过程成为可能.目前,这些技术已经广泛应用到DSB研究领域,促进了多个关键修复因子功能的解析及多种体系下断裂热点的鉴定,加深了在染色体环境对DSB调控机制方面的理解,让DSB的研究进入了 一个新维度.结合实验室的相关研究内容,该文将对DSB的产生、修复机制及目前已发表的DSB高通量检测技术进行简要概述和总结.
Abstract
The DSB(DNA double-strand break)is the most severe type of DNA damage.Studying the underlying mechanisms of DSB generation and repair will help to understand disease initiation and progression.In the past decade,the advent of next-generation sequencing has greatly promoted the development of sequencing-based methods for DSB detection,making it possible to identify the"hotspots"of DSB and measure the repair and processing events.These assays have been widely applied to investigate the roles of many key repair factors,DSB hotspots in different biological contexts,impact of chromatin context on DSB repair,opening a fascinating portal into DSB research.Combined with the laboratory's research focus,this article will briefly summarize the sources of DSB,their repair pathways,and published genome-wide detection assays for DSB.
关键词
DNA损伤修复/DNA双链断裂/高通量测序Key words
DNA damage and repair/DNA double-strand break/high throughput sequencing引用本文复制引用
出版年
2024
NETL
NSTL
万方数据