Expression of LINC01128 in Endometrial Cancer Tissue and Its Effect on Cell Malignant Progression by Regulating miR-367-3p/KLF5 Axis
This study aims to investigate the expression of LINC01128 in EC(endometrial cancer)tissue and its effect on cell malignant progression by regulating the miR-367-3p/KLF5(Krüppel like factor 5)axis.qRT-PCR was ap-plied to detect the expression levels of LINC01128,miR-367-3p,and KLF5 mRNA in EC tissue,cell line HEC-1 A,human endometrial epithelial cells CP-H058.HEC-1A cells cultured in vitro were randomly separated into five groups:NC group(normal culture),si-LINC01128 group,si-NC group,miR-367-3p mimic group,and miR-NC group.And the si-NC group,si-LINC01128 group,miR-367-3p mimic group,and miR-NC group were transfected with si-NC,si-LINC01128,miR-367-3p mimic,and miR-NC,respectively.CCK-8 method and TUNEL staining were applied to detect the proliferation and apoptosis of HEC-1A cells,respectively.Transwell and scratch experiments were applied to detect the invasion and migra-tion of HEC-1A cells,respectively.Dual luciferase reporter gene experiment was applied to verify the targeting relationship between LINC01128 and miR-367-3p,and between miR-367-3p and KLF5.Western blot was applied to detect the ex-pression of KLF5 in cells.Compared with adjacent tissues,the expression of LINC01128 and KLF5 mRNA in EC tissues was increased,while miR-367-3p expression was decreased;compared with CP-H058 cell,the expression of LINC01128 and KLF5 mRNA in EC cell HEC-1 A was increased,while miR-367-3p expression was reduced(P＜0.05).There was no significant difference in the expression of LINC01128,miR-367-3p,KLF5 mRNA,as well as HEC-1 A cell proliferation,apoptosis,migration,and invasion between the NC group and the si-NC group(P＞0.05).Compared with the si-NC group,the expression of LINC01128 and KLF5 mRNA,D450 value,migration rate,invasion number,and Bcl-2 expression in the si-LINC01128 group were all reduced,while miR-367-3p expression,apoptosis rate,and Bax expression were all increased(P＜0.05).There was no great difference in the expression of LINC01128,miR-367-3p,KLF5 mRNA,as well as HEC-1A cell proliferation,apoptosis,migration,and invasion between the NC group and the miR-NC group(P＞0.05).Compared with the miR-NC group,there was no great difference in LINC01128 expression in the miR-367-3p mimic group(P＞0.05),the KLF5 mRNA expression,D450 value,migration rate,invasion number,and Bcl-2 expression were all reduced,while miR-367-3p expression,apoptosis rate,and Bax expression were all increased(P＜0.05).The results of dual luciferase reporter gene experiment showed that miR-367-3p had targeted regulatory relationship with LINC01128 and KLF5.LINC01128 is highly expressed in EC tissues and cells,and interference with LINC01128 can inhibit malignant progres-sion of EC by regulating the miR-367-3p/KLF5 axis.
endometrial cancerLINC01128miR-367-3pKrüppel like factor 5malignant progression
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