Gliomas are the most common malignant tumors of the central nervous system and face chal-lenges such as resistance from heat shock proteins and obstacles posed by the blood-brain barrier during low-temperature photothermal therapy.In this study,a novel gene delivery system,aT@FVNPs,is synthesized by modi-fying T cell membranes with photothermal agents and HSP90-siRNA on the surface of carriers.This system aims to achieve effective low-temperature photothermal therapy for gliomas.The basic properties of the carriers were determined using a nanoparticle size analyzer,UV-visible spectrophotometer,and fluorescence spectrometer.The performance of aT@FVNPs in penetrating endothelial cells and the ability to knock down HSP90 and kill tumor cells in vitro were assessed using mouse endothelial cells(Bend.3)and glioma cells(GL261).The results showed that aT@FVNPs more readily crossed the endothelial cell barrier and induced stronger glioma cell killing under low-temperature photothermal conditions due to HSP90 knockdown.Additionally,using a mouse orthotopic glioma model,the in vivo targeting potential of aT@FVNPs was further evaluated,demonstrating a stronger accumulation at the glioma site compared to the FVNPs group.With laser irradiation,aT@FVNPs group showed more obvious pathological necrosis and induced cell apoptosis.In summary,aT@FVNPs can cross the blood-brain barrier and target glioma sites,effectively killing tumor cells under low-temperature photothermal conditions by inhibiting heat shock protein expression.
关键词
脑胶质瘤/低温光热治疗/热休克蛋白/血脑屏障/基因递送系统
Key words
glioma/low-temperature photothermal therapy/heat shock proteins/blood-brain barrier/gene delivery system
维普
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