Effects of LncRNA MIF-AS1 on the Proliferation,Migration,and Invasion of Esophageal Squamous Cell Carcinoma Cells by Regulating the miR-423-5p/KDM2A Axis
This study aims to investigate the effects of LncRNA(long non-coding RNA)MIF-AS1 on the proliferation,migration,and invasion of ESCC(esophageal squamous cell carcinoma)cells by regulating the miR-423-5p(microRNA-423-5p)/KDM2A(histone demethylase 2A)axis.qRT-PCR was applied to detect the expression of LncRNA MIF-AS1,miR-423-5p,and KDM2A mRNA in ESCC tissues and adjacent tissues of 39 ESCC patients who underwent surgery in Pangang Group General Hospital from January 2023 to January 2024.KYSE30 cells were separated into Control group,sh-NC group,sh-MIF-AS1 group,sh-MIF-AS1+anti-NC group,and sh-MIF-AS1+anti miR-423-5p group randomly.The mRNA expression of LncRNA MIF-AS1,miR-423-5p,and KDM2A was detected in KYSE30 cells.Plate cloning,scratch assay,and Transwell assay were applied to detect the prolif-eration,migration,and invasion of KYSE30 cells,respectively.Western blot was applied to detect the expression of PCNA,KDM2A,and MMP-9 proteins in KYSE30 cells.The results showed that the expression of LncRNA MIF-AS1 and KDM2A mRNA in ESCC tissues was higher than that in adjacent tissues,while the expression of miR-423-5p was lower than that in adjacent tissues(P＜0.05).Silencing LncRNA MIIF-AS1 resulted in decreased cell clone number,scratch healing rate,invasion number,LncRNA MIIF-AS1,PCNA protein,KDM2A mRNA and KD-M2A protein,and MMP-9 protein expression.Inhibition of miR-423-5p reversed the effect of silencing LncRNA MIF-AS1 on the above indexes(P＜0.05).LncRNA MIF-AS1 could target negative regulation of miR-423-5p,and miR-423-5p could target negative regulation of KDM2A.These results suggest that silencing LncRNA MIF-AS1 can inhibit the proliferation,migration,and invasion of ESCC cells,and its mechanism may be achieved by regulat-ing the miR-423-5p/KDM2A signaling pathway.
long non-coding RNA MIF-AS1microRNA-423-5phistone demethylase 2Aesophageal squamous cell carcinomaproliferationmigration and invasion
万方数据
维普
