摘要
该文探讨了长链非编码RNA(LncRNA)MIF-AS1调节微小RNA-423-5p(miR-423-5p)/组蛋白去甲基化酶2A(KDM2A)轴对食管鳞状细胞癌(ESCC)细胞增殖、迁移和侵袭的影响.qRT-PCR法检测39例2023年1月至2024年1月期间在攀钢集团总医院进行手术的ESCC患者术中切除的ESCC组织及癌旁组织中 LncRNA MIF-AS1、miR-423-5p、KDM2A mRNA的表达.将KYSE30细胞随机分为 Control组、sh-NC组、sh-MIF-AS1组、sh-MIF-AS1+anti-NC组、sh-MIF-AS1+anti-miR-423-5p组,检测KYSE30细胞中LncRNA MIF-AS1、miR-423-5p、KDM2A mRNA表达情况;平板克隆法、划痕实验、Transwell实验分别检测KYSE30细胞增殖、迁移、侵袭情况;Western blot检测KYSE30细胞中PCNA、KDM2A、MMP-9蛋白的表达水平.结果显示,ESCC组织LncRNA MIF-AS1、KDM2A mRNA表达水平高于癌旁组织,miR-423-5p表达水平低于癌旁组织(P＜0.05).沉默LncRNA MIF-AS1导致细胞克隆数、划痕愈合率、侵袭数、LncRNA MIF-AS1、PCNA蛋白、KDM2A mRNA和KDM2A蛋白、MMP-9蛋白表达水平降低(P＜0.05);抑制miR-423-5p逆转了沉默LncRNA MIF-AS1对上述指标的影响(P＜0.05).LncRNA MIF-AS1可以靶向负调控miR-423-5p,miR-423-5p可以靶向负调控KDM2A.以上结果表明,沉默LncRNA MIF-AS1可以抑制ESCC细胞的增殖、迁移、侵袭,其机制可能是通过调控miR-423-5p/KDM2A信号通路实现的.
Abstract
This study aims to investigate the effects of LncRNA(long non-coding RNA)MIF-AS1 on the proliferation,migration,and invasion of ESCC(esophageal squamous cell carcinoma)cells by regulating the miR-423-5p(microRNA-423-5p)/KDM2A(histone demethylase 2A)axis.qRT-PCR was applied to detect the expression of LncRNA MIF-AS1,miR-423-5p,and KDM2A mRNA in ESCC tissues and adjacent tissues of 39 ESCC patients who underwent surgery in Pangang Group General Hospital from January 2023 to January 2024.KYSE30 cells were separated into Control group,sh-NC group,sh-MIF-AS1 group,sh-MIF-AS1+anti-NC group,and sh-MIF-AS1+anti miR-423-5p group randomly.The mRNA expression of LncRNA MIF-AS1,miR-423-5p,and KDM2A was detected in KYSE30 cells.Plate cloning,scratch assay,and Transwell assay were applied to detect the prolif-eration,migration,and invasion of KYSE30 cells,respectively.Western blot was applied to detect the expression of PCNA,KDM2A,and MMP-9 proteins in KYSE30 cells.The results showed that the expression of LncRNA MIF-AS1 and KDM2A mRNA in ESCC tissues was higher than that in adjacent tissues,while the expression of miR-423-5p was lower than that in adjacent tissues(P＜0.05).Silencing LncRNA MIIF-AS1 resulted in decreased cell clone number,scratch healing rate,invasion number,LncRNA MIIF-AS1,PCNA protein,KDM2A mRNA and KD-M2A protein,and MMP-9 protein expression.Inhibition of miR-423-5p reversed the effect of silencing LncRNA MIF-AS1 on the above indexes(P＜0.05).LncRNA MIF-AS1 could target negative regulation of miR-423-5p,and miR-423-5p could target negative regulation of KDM2A.These results suggest that silencing LncRNA MIF-AS1 can inhibit the proliferation,migration,and invasion of ESCC cells,and its mechanism may be achieved by regulat-ing the miR-423-5p/KDM2A signaling pathway.
维普
万方数据