CircDOCK1 Affects Gastric Cancer Progression by Regulating the miR-128-3p/HMGB3 Axis
This article aims to explore the effect of circDOCK1 on gastric cancer cell functions and its mechanism.The expression levels of circDOCK1,miR-128-3p and HMGB3 in gastric cancer tissues,adjacent tis-sues,human gastric mucosal epithelial cells GES-1,and human gastric cancer cells HGC-27 were determined by qRT-PCR and Western blot.si-NC,si-circDOCK1,miR-NC,miR-128-3p mimic,si-circDOCK1+miR-NC inhibitor and si-circDOCK1+miR-128-3p inhibitor were transfected into HGC-27 cells,respectively.The HGC-27 cells were divided into si-NC group,si-circDOCK1 group,miR-NC group,miR-128-3p mimic group,si-circDOCK1+miR-NC inhibitor group and si-circDOCK1+miR-128-3p inhibitor group.Targeting relationship of circDOCK1 and miR-128-3p was assessed by dual-luciferase reporter assay.CCK-8 method,clone formation experiment,scratch experiment,Transwell experiment and flow cytometry were used to detect cell functions.CircDOCK1 and HMGB3 levels were upregulated,while miR-128-3p level was downregulated in gastric cancer tissues and HGC-27 cells(P＜0.05).CircDOCK1 could target miR-128-3p to regulate HMGB3 expression.Transfection of si-circDOCK1 or miR-128-3p mimic promoted cell apoptosis(P＜0.05),but inhibited cell proliferation,migration and invasion(P＜0.05).In addition,miR-128-3p inhibitor reversed the promoting effect of si-circDOCK1 on HGC-27 cell apop-tosis,as well as the inhibitory effect on cell proliferation,migration and invasion.CircDOCK1 promoted gastric cancer cell proliferation,migration,invasion,and induced apoptosis by miR-128-3p/HMGB3 axis,indicating that circDOCK1 might be a potential molecular target for the treatment of gastric cancer.
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