A comparison of methods for identifying mast cells of Cyprinus carpio
Objective To compare different identifying methods of the mast cells of red crucian carp (Cyprinus carpio).Methods The gill filaments were taken from Cyprinus carpio infected with Edwardsiella tarda and fixed in Bouin's solution.The hematoxylin-eosin (HE),toluidine blue (TB),Alcian blue (AB),neutral red,Masson trichrome,May-Grünwald Giemsa (MGG) stainings and streptavidin-biotin complex (SABC) immunohistochemical method were used to observe the mast cells.The smears of head kidney stained by Wright-Giemsa were used as a supplementary method.Results AB and TB were desirable staining solutions for clearly displaying mast cells,and MGG staining came second.Immunohistochemical method showed the small number of tryptase-positive mast cells and a weak positive reaction.The cells in smears were dispersed and showed different developmental stages.Conclusion The immunohistochemistry and the histochemical staining combined with Wright-Giemsa-stained smears are two good ways to identify the mast cells of fish.
NETL
NSTL
万方数据
维普
