CD226在小鼠小肠3型固有淋巴样细胞(ILC3)中的表达
Expression of CD226 in the small intestinal group 3 innate lymphoid cells(ILC3)in mice
羊璐 1马菁昌 2刘懿天 2王婷婷 1王玉玲 2庄然 2张正祥1
作者信息
- 1. 延安大学医学院,陕西延安 716000
- 2. 空军军医大学基础医学院免疫学教研室,陕西西安 710032
- 折叠
摘要
目的 观察CD226在小肠3型固有淋巴样细胞(ILC3)上的表达情况.方法 采用生物信息学方法分析小鼠CD226在ILC中的表达.分离正常C57BL/6J小鼠小肠黏膜固有层淋巴细胞(LPL),流式细胞术检测CD226在ILC1和ILC3上的表达.构建葡聚糖硫酸钠(DSS)诱导小鼠肠炎模型,观察CD226在ILC3上表达的变化情况.结果 小鼠小肠ILC1和ILC3均表达CD226;肠炎模型中ILC3所占比例降低,但CD226在ILC3上的表达水平升高.结论 小鼠小肠表达CD226,DSS诱导的肠炎模型中虽然ILC3比例降低,但CD226在ILC3的表达增加.
Abstract
Objective To observe the expression of adhesion molecule CD226 on the small intestinal group 3 innate lymphoid cells(ILC3)in mice.Methods The bioinformatics was used to analyze the expression of CD226 on murine ILCs.Small intestinal mucosal lamina propria lymphocytes(LPL)were isolated from wild-type C57BL/6J mice,and the expression of CD226 on ILC1 and ILC3 was detected by flow cytometry.A mouse model of dextran sulfate sodium(DSS)-induced colitis was constructed to observe the changes in the expression of CD226 on ILC3.Results Both ILC1 and ILC3 in the mice small intestine expressed CD226 molecules;the proportion of ILC3 was reduced,while the expression level of CD226 on ILC3 was increased in the colitis model.Conclusion CD226 is expressed on the small intestines of mice,and although the proportion of ILC3 decreases in the DSS-induced colitis,the expression of CD226 on ILC3 increases.
关键词
3型固有淋巴样细胞(ILC3)/CD226/黏膜免疫/肠道Key words
group 3 innate lymphoid cells(ILC3)/CD226/mucosal immunity/intestinal tract引用本文复制引用
基金项目
国家自然科学基金(81871258)
延安大学科研计划项目(YDY2020-21)
出版年
2024
NETL
NSTL
万方数据