miR-185-5p alleviates the inflammatory response of acute gouty arthritis by inhibiting of IL-1β
Objective To investigate the relationship between interleukin-1β(IL-1β)and miR-185-5p in the process of joint injury in acute gouty arthritis(AGA).Methods The serum miR-185-5p levels of 89 AGA patients and 91 healthy volunteers were detected by real-time quantitative PCR.The correlation between miR-185-5p expression level and VAS score or IL-1β expression level was evaluated by Pearson correlation coefficient method.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of miR-185-5p in AGA.THP-1 cells were induced by sodium urate(MSU)to construct an in vitro acute gouty inflammatory cell model.After the expression level of miR-185-5p in THP-1 cells was upregulated or downregulated by transfection of miR-185-5p mimics or inhibitors in vitro,inflammatory cytokines of THP-1 cells,such as IL-1β,IL-8 and tumor necrosis factor α(TNF-α),were detected by ELISA.The luciferase reporter gene assay was used to determine the interaction between miR-185-5p and the 3'-UTR of IL-1β.Results Compared with the healthy control group,the expression level of serum miR-185-5p in AGA patients was significantly reduced.The level of serum miR-185-5p was negatively correlated with VAS score and IL-1β expression level.The area under the curve(AUC)was 0.905,the sensitivity was 80.17％and the specificity was 83.52％.Down-regulation of miR-185-5p significantly promoted the expression of IL-1β,IL-8 and tumor necrosis factor(TNF-α),while overexpression of miR-185-5p showed the opposite results.Luciferase reporter gene assay showed that IL-1β was the target gene of miR-185-5p,and miR-185-5p negatively regulated the expression of IL-1β.Conclusion miR-185-5p alleviates the inflammatory response in AGA by inhibiting IL-1β.
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