首页|强直性脊柱炎患者外周血单个核细胞微小RNA的差异表达及其与免疫炎症反应的相关性

强直性脊柱炎患者外周血单个核细胞微小RNA的差异表达及其与免疫炎症反应的相关性

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  • NETL
  • NSTL
  • 万方数据
目的 检测强直性脊柱炎(AS)患者外周血单个核细胞(PBMC)中微小RNA(miRNA)的表达及其与免疫炎症反应的关系.方法 选取AS患者(AS组)及健康志愿者(对照组),每组15例.利用高通量测序技术筛选PBMC中miRNA表达,利用实时定量PCR检测6个差异表达miRNA水平,ELISA检测外周血细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-17、IL-23表达,Spearman法分析差异miRNA与疾病活动、免疫炎症指标的相关性.结果 与对照组相比,AS患者组共有44个miRNA差异表达,其中有22个上调,22个下调(差异倍数≥1);其中miR-1-3p、miR-133a-5p表达明显上调,miR-127-5p、miR-345-3p、miR-136-3p 表达明显下调;且 AS 患者 TNF-α、IL-1β、IL-17、IL-23 表达明显升高,miR-1-3p 与 TNF-α、C 反应蛋白(CRP)、Bath强直性脊柱炎疾病活动性指数(BASDAI)呈正相关,miR-133a-5p与TNF-α呈正相关,miR-127-5p与红细胞沉降率(ESR)、脊柱疼痛视觉模拟评分法(VAS)评分呈负相关,miR-345-3p与IL-17呈负相关,miR-136-3p与IL-17、BASDAI呈负相关.结论 AS患者PBMC中miRNA异常表达,差异表达miRNA与患者疾病活动及免疫炎症指标存在相关性.
Differentially expressed microRNAs in peripheral blood mononuclear cells of ankylosing spondylitis patients and its correlation with immune inflammatory response
Objective To investigate the differentially expressed miRNAs in peripheral blood mononuclear cells(PBMCs)of ankylosing spondylitis(AS)patients,and explore its relevance with the immune inflammatory responses.Methods Fifteen AS patients(AS group)and fifteen healthy volunteers(control group)were recruited in this research.High-throughput RNA sequencing was used to screen miRNA expression in PBMCs.Real-time quantitative PCR was used to detect the six differentially expressed miRNAs.ELISA was applied to test the levels of proinflammatory cytokines,such as TNF-α,IL-1β,IL-17,and IL-23.Finally,Spearman correlation analysis was conducted to study the correlations of differentially expressed miRNAs with disease activity indicators and immune inflammatory markers.Results Forty-four miRNAs were significantly differentially expressed in AS patients,manifested as 22 up-regulated and 22 down-regulated(fold change≥1).Among them,miR-1-3p and miR-133a-5p were up-regulated obviously,while miR-127-5p,miR-345-3p and miR-136-3p were down-regulated significantly.TNF-α,IL-1β,IL-17 and IL-23 were significantly increased simultaneously in AS patients.Moreover,miR-1-3p was positively correlated with TNF-α,CRP and BASDAI score;miR-133a-5p was positively correlated with TNF-α;miR-127-5p was negatively correlated with ESR and VAS;miR-345-3p was negatively correlated with IL-17;miR-136-3p was negatively correlated with IL-17 and BASDAI score.Conclusion The miRNAs are abnormally expressed in PBMCs of AS patients,and the differentially expressed miRNAs are associated with disease activity indicators and immune inflammatory cytokines.

ankylosing spondylitismicroRNAhigh-throughput RNA sequencingcytokinesimmune inflammation

黄旦、刘健、汪元、曹云祥、万磊、张皖东、方妍妍

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安徽中医药大学第一附属医院风湿科,安徽合肥 230031

安徽省中医科学院风湿病研究所,安徽合肥 230031

强直性脊柱炎(AS) 微小RNA(miRNA) 高通量测序 细胞因子 免疫炎症

国家自然科学基金安徽省高等学校自然科学研究重点项目现代中医内科应用基础与开发研究安徽省重点实验室开放基金(2021)现代中医内科应用基础与开发研究安徽省重点实验室开放基金(2021)新安医学教育部重点实验室开放基金安徽省重点实验室项目安徽省"115"创新团队项目(第十二批)

82205054KJ2021A05582021AKLMCM0022021AKLMCM0032020xayx081606c08238皖人才办[2019]1号

2024

10.13423/j.cnki.cjcmi.009681

细胞与分子免疫学杂志
中国免疫学会,第四军医大学

细胞与分子免疫学杂志

CSTPCD北大核心
影响因子：0.817
ISSN：1007-8738
年,卷(期)：2024.40(2)
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