系统性红斑狼疮患者外周血CD19+CD25+Breg比例和细胞表面PD-1和PD-L1的表达及其与临床指标的相关性

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中文摘要：目的研究程序性死亡蛋白1(PD-1)与其配体PD-L1在系统性红斑狼疮(SLE)患者外周血CD19+CD25+调节性B细胞(Breg)的表达及临床意义.方法收集50例SLE患者和41例健康人(HC)外周血标本,采用流式细胞仪检测外周血中CD19+CD25+Breg的比例,以及CD19+CD25+Breg的PD-1、PD-L1表达,同时采集患者的临床表现和实验室指标等临床信息.使用免疫磁珠分选CD4+T细胞与CD19+B细胞,体外细胞共培养,检测Breg的分化.结果 SLE患者活动组外周血中CD19+CD25+Breg比例低于HC,CD25+B细胞PD-1、PD-L1的表达高于HC;SLE患者有胸腔积液、关节炎、C反应蛋白(CRP)上升的患者Breg频率高于对应阴性组;SLE患者有IgM下降、抗核糖核蛋白(RNP)抗体阳性的患者Breg频率低于对应阴性组;SLE患者有感染、发热、关节炎、IgA上升的患者CD19+CD25+PD-1+细胞频率高于对应阴性组;SLE患者有感染、发热、IgA上升的患者CD19+CD25+PD-L1+细胞频率高于对应阴性组;活化的CD4+T细胞有利于CD19+B细胞表面CD25的表达.结论 SLE患者外周血中CD19+CD25+Breg降低,但细胞表面PD-1与PD-L1的表达增加,并且与相关临床表现及实验室参数存在一定的关系.活化的CD4+T细胞促进Breg的分化.

外文标题：The proportion of CD19+CD25+Bregs in peripheral blood and the expression of PD-1 and PD-L1 on cell surface in patients with systemic lupus erythematosus and their correlation with clinical indicators

外文摘要：Objective To investigate the expression and clinical significance of PD-1 and its ligand PD-L1 in peripheral blood CD19+CD25+regulatory B cells(Bregs)in patients with systemic lupus erythematosus(SLE).Methods Peripheral blood samples were collected from 50 patients and 41 healthy controls(HCs).The proportion of CD19+CD25+Bregs in peripheral blood as well as the expression of PD-1+B and PD-L1+B cells on CD19+CD25+/-B cells,were detected by flow cytometry.At the same time,clinical information,such as clinical manifestations and laboratory indexes,was collected from patients.CD4+T cells and CD19+B cells were isolated by immunomagnetic beads and co-cultured in vitro to detect the differentiation of Bregs.Results The proportion of CD19+CD25+Bregs in the peripheral blood of SLE patients was lower than that in HC,while the expression of PD-1 and PD-L1 on Bregs was higher than that in HCs.SLE patients with pleural effusion,arthritis,and elevated CRP had a higher frequency of Bregs compared to the corresponding negative group.SLE patients with decreased immunoglobulin M(IgM)and positive anti-ribonuclear protein(RNP)antibodies had a lower frequency of Bregs compared to the corresponding negative group.SLE patients with infection,fever,arthritis,and elevated immunoglobulin A(IgA)had a higher frequency of CD19+CD25+PD-1+cells compared to the corresponding negative group.SLE patients with infection,fever,and elevated IgA had a higher frequency of CD19+CD25+PD-L1+cells compared to the corresponding negative group.And activated CD4+T cells were beneficial to the expression of CD25 on CD19+B cells.Conclusion The peripheral blood CD19+CD25+Bregs are decreased in SLE patients,while the expression of PD-1 and PD-L1 on cell surface is increased,which is correlated with clinical manifestations and laboratory parameters.Activation of CD4+T cells promotes the differentiation of Bregs.

外文关键词：

systemic lupus erythematosus(SLE)CD19+CD25+BregPD-1PD-L1

作者：

谢卓贝、代丽、何豪华、洪登校、王元元、徐文艳、陈中新、李柏青、谢长好

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作者单位：

蚌埠医科大学第一附属医院风湿免疫科,安徽蚌埠 233000

徐州医科大学第二附属医院老年科,江苏徐州 221000

蚌埠医科大学组织与胚胎学教研室

蚌埠医科大学第一附属医院检验科

慢性疾病免疫学基础与临床安徽省重点实验室

炎症相关性疾病基础与转化研究安徽省重点实验室,安徽蚌埠 233000

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关键词：

系统性红斑狼疮(SLE) CD19+CD25+调节性B细胞(Breg) 程序性死亡蛋白1(PD-1) 程序性死亡蛋白1配体1(PD-L1)

基金：

安徽省自然科学研究基金面上项目安徽省高等学校自然科学研究重点项目蚌埠医学院研究生科研创新计划蚌埠医学院研究生科研创新计划蚌埠医学院研究生科研创新计划

项目编号：

2108085MH258KJ2021A0763Byycx21064Byycxz21093Byycx22028

出版年：

2024

DOI：

10.13423/j.cnki.cjcmi.009691

细胞与分子免疫学杂志

中国免疫学会,第四军医大学

细胞与分子免疫学杂志

CSTPCD北大核心

影响因子：0.817

ISSN：1007-8738

年,卷(期)：2024.40(2)

参考文献量25