The functional differences of macrophages derived from murine bone marrow and peritoneal cavity
Objective To compare the functional differences between bone marrow derived macrophages and peritoneal macrophages,which may provide the basis for the selection of macrophages in immunological research and immunoregulatory drug evaluation.Methods Marophage colony-stimulating factor(M-CSF)was used to induce the differentiation of bone marrow monocytes into macrophages,and thioglycolate medium was used to induce peritonitis to obtain peritoneal macrophages.After both macrophages being stimulated by zymosan,LPS,R848 and CpG respectively,mRNA levels of tumor necrosis factor α(TNF-α),interleukin 6(IL-6),macrophage inflammatory protein 1α(MIP-1α),monocyte chemoattractant protein 1(MCP-1)were measured by Real-time fluorescent quantitative PCR and the concentrations of secreted TNF-α,IL-6,MI P-1α and MCP-1 were detected by ELISA.In addition,the expression of costimulatory molecules CD80,CD86,CD40 and histocompatibility complex Ⅱ(MHCⅡ)on the cell surface was analyzed by flow cytometry.Results After inducing by different TLR ligands,mRNA expression levels of inflammatory cytokines and chemokines were increased in both macrophages.The secretion of TNF-α,IL-6,MIP-1α and MCP-1 in peritoneal macrophages and the expression of CD86 and MHC Ⅱ on the surface of peritoneal macrophages were significantly higher than those of bone marrow derived macrophages.Conclusion There are significant differences in the expression of inflammatory factors,chemokines,costimulatory molecules,and histocompatibility complex between bone marrow derived macrophages and peritoneal macrophages.Peritoneal macrophages have more complete macrophage function and is more suitable for immunological research and immunomodulatory drug evaluation.
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