Expression characteristics and clinical significance of B7-H3 in colorectal cancer
Objective To elucidate the expression characteristics and clinical significance of B7 homolog 3(B7-H3)in colorectal cancer(CRC)and to explore its associations with tumor glycolysis and immune cell infiltration in the tumor microenvironment.Methods The transcriptomic and clinicopathological data of CRC were obtained from the TCGA database and analyzed to determine the expression and clinical relevance of B7-H3.Correlations between glycolysis-related genes and B7-H3 expression were assessed based on TCGA data.The associations between B7-H3 expression and the infiltration of 22 types of immune cells were analyzed using the CIBERSORT algorithm.Immunohistochemical staining was used to verify the results of database analysis in surgical specimens and adjacent normal tissue specimens of 51 CRC patients.Results B7-H3 was significantly upregulated in CRC tissues and demonstrated strong correlations with tumor invasion depth,advanced TNM stage,and poor prognosis.Additionally,B7-H3 expression was closely associated with the upregulation of glycolysis-related genes in CRC.Immune cell infiltration analysis revealed that a total of 16 types of immune cells were significantly correlated with B7-H3 expression.Furthermore,an inverse relationship between B7-H3 expression and CD8+T cell infiltration was identified at the transcriptional level but not at the protein level.Conclusion B7-H3 is highly expressed in CRC tissues and is significantly correlated with disease progression and poor prognosis.Furthermore,the association of B7-H3 expression with glycolysis-related genes and immune cell infiltration suggests a pivotal role of B7-H3 in the regulation of tumor glycolysis and cancer immunity.
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