The expression of T cell costimulatory molecules and the proportion of NK cells in peripheral blood of patients with pulmonary tuberculosis
Objective To investigate the levels of costimulator molecules CD28,CD152/CTLA4,PD-1 and NK cells in peripheral blood of patients with pulmonary tuberculosis(PTB),and to explore the activation of T cell subsets and function of NK cell in PTB patients,as well as the role of T cell costimulatory signaling molecules in the pathogenesis of PTB.Methods Thirty-two PTB patients(PTB group)and 15 health examiners(control group)were recruited.The expression of CD28 and CD152 on peripheral blood T lymphocytes was detected by flow cytometry.The relationship between the two group was analyzed using receiver operating characteristic(ROC)curves.The expression of PD-1 on regulatory T cells(Tregs)and the proportion of NK cells in peripheral blood were detected by flow cytometry.Results Compared with the control group,the proportions of CD8+CD28+T cells and CD8+CD152+T cells were significantly lower in the PTB group.The ROC curve showed that the variable CD8+CD152+T cell proportion had some predictive value in PTB(AUC=0.800,Cl=0.664-0.936).The proportions of CD4+CD28+T cells and CD4+CD152+T cells had no predictive value.There was a positive correlation between CD4+CD28+T cells and CD8+CD28+T cells in PTB group(r=0.563).Compared with the control group,the proportion of NK cells was significantly reduced in the PTB group.Conclusion The proportions of CD8+CD152+T cells,CD8+CD28+T cells,and NK cells significantly reduced in PTB patients.
万方数据
维普
