Serum from Xinfeng Capsule-treated rats affect the proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis by regulating circular RNA Cbl proto-oncogene B(circ-CBLB)
Serum from Xinfeng Capsule-treated rats affect the proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis by regulating circular RNA Cbl proto-oncogene B(circ-CBLB)
Objective To explore the effect of serum from Xinfeng Capsule(XFC)-treated rats on the proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis(RA-FLS)by regulating the circular RNA Cbl oncogene B(circ-CBLB).Methods XFC was administered orally to rats to prepare drug-containing serum.Human RA-FLS were stimulated with 100 μL of 10 ng/mL tumor necrosis factor-alpha(TNF-α)to establish the model.pcDNA3.1-circ-CBLB and negative control were constructed and transfected into RA-FLS.The experiment was divided into six groups:control group,TNF-αtreated RA-FLS group,XFC treated RA-FLS group,pcDNA3.1-circ-CBLB-NC,pcDNA3.1-circ-CBLB(overexpression group),pcDNA3.1-cicr-CBLB combined with XFC treated group(overexpression+XFC group).Cell viability was assessed by CCK-8 assay;cell cycle and apoptosis by flow cytometry,and the expression levels of circ-CBLB in each group by real-time quantitative PCR.The levels of inflammatory cytokines interleukin 4(IL-4)、IL-10、IL-6 and TNF-α were measured by ELISA.Results The optimal serum was 200 mL/L,and the treatment time was 72 hours;Compared with the model group at the same time point,the cell viability of XFC group,overexpression group,and overexpression+XFC group were lower,while the expression level and apoptosis rate of circ-CBLB were higher.The proportion of cells in S phase and G2 phase was higher.Additionally,the levels of IL-4 and IL-10 were higher,while the levels of IL-6 and TNF-α were lower.Conclusion XFC treatment upregulates the expression of circ-CBLB in RA-FLS,increases anti-inflammatory cytokines,decreases pro-inflammatory cytokines,inhibits the viability of RA-FLS,increases apoptosis rate,extends the cell cycle,suppresses the proliferation of RA-FLS,and promotes its apoptosis.
维普
万方数据