首页|新风胶囊含药血清通过调控环状RNA Cbl原癌基因B(circ-CBLB)影响类风湿关节炎滑膜成纤维细胞增殖和凋亡

新风胶囊含药血清通过调控环状RNA Cbl原癌基因B(circ-CBLB)影响类风湿关节炎滑膜成纤维细胞增殖和凋亡

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目的 探讨新风胶囊(XFC)含药血清通过调控环状RNA Cbl原癌基因B(circ-CBLB)对类风湿关节炎成纤维细胞样滑膜细胞(RA-FLS)增殖、凋亡的影响。方法 XFC灌胃大鼠，制备含药血清。人RA-FLS来源于RA患者滑膜组织，用100 μL的10 ng/mL肿瘤坏死因子α(TNF-α)刺激RA-FLS建立模型。构建pcDNA3。1-circ-CBLB及阴性对照，分别转染至RA-FLS中。实验分为对照组、TNF-α处理的RA-FLS组、XFC处理的RA-FLS组、pcDNA3。1-circ-CBLB-NC转染的RA-FLS组(过表达空转组)、pcDNA3。1-circ-CBLB转染的RA-FLS组(过表达组)、pcDNA3。1-circ-CBLB联合XFC处理的RA-FLS组(过表达给药组)。采用CCK-8法检测细胞活力，采用流式细胞术检测细胞周期与凋亡，采用实时定量PCR检测circ-CBLB表达水平，采用ELISA检测抗炎因子白细胞介素4(IL-4)、IL-10，促炎因子IL-6、TNF-α水平。结果 XFC最佳含药血清量为200 mL/L，处理时间为72 h;与同一时间模型组相比，XFC组、过表达组、过表达给药组的细胞活力均下降。与模型组比较，XFC组、过表达组、过表达给药组circ-CBLB表达水平升高、凋亡率升高，S期和G2期细胞比例增加，IL-4、IL-10含量升高，IL-6、TNF-α含量降低。结论 XFC处理上调RA-FLS中circ-CBLB表达，提高抗炎因子的水平，降低促炎因子的水平，抑制RA-FLS的细胞活力，提高其凋亡率，延长细胞周期，抑制RA-FLS增殖并促进其凋亡。
Serum from Xinfeng Capsule-treated rats affect the proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis by regulating circular RNA Cbl proto-oncogene B(circ-CBLB)
Objective To explore the effect of serum from Xinfeng Capsule(XFC)-treated rats on the proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis(RA-FLS)by regulating the circular RNA Cbl oncogene B(circ-CBLB).Methods XFC was administered orally to rats to prepare drug-containing serum.Human RA-FLS were stimulated with 100 μL of 10 ng/mL tumor necrosis factor-alpha(TNF-α)to establish the model.pcDNA3.1-circ-CBLB and negative control were constructed and transfected into RA-FLS.The experiment was divided into six groups:control group,TNF-αtreated RA-FLS group,XFC treated RA-FLS group,pcDNA3.1-circ-CBLB-NC,pcDNA3.1-circ-CBLB(overexpression group),pcDNA3.1-cicr-CBLB combined with XFC treated group(overexpression+XFC group).Cell viability was assessed by CCK-8 assay;cell cycle and apoptosis by flow cytometry,and the expression levels of circ-CBLB in each group by real-time quantitative PCR.The levels of inflammatory cytokines interleukin 4(IL-4)、IL-10、IL-6 and TNF-α were measured by ELISA.Results The optimal serum was 200 mL/L,and the treatment time was 72 hours;Compared with the model group at the same time point,the cell viability of XFC group,overexpression group,and overexpression+XFC group were lower,while the expression level and apoptosis rate of circ-CBLB were higher.The proportion of cells in S phase and G2 phase was higher.Additionally,the levels of IL-4 and IL-10 were higher,while the levels of IL-6 and TNF-α were lower.Conclusion XFC treatment upregulates the expression of circ-CBLB in RA-FLS,increases anti-inflammatory cytokines,decreases pro-inflammatory cytokines,inhibits the viability of RA-FLS,increases apoptosis rate,extends the cell cycle,suppresses the proliferation of RA-FLS,and promotes its apoptosis.

Xinfeng Capsule(XFC)circular RNA Cbl proto-oncogene B(circ-CBLB)fibroblast-like synoviocytes(FLS)cell proliferationcell apoptosiscell factor

李舒、万磊、刘健、黄传兵、李方泽、程静、胡赛赛、陈莹莹、朱子衡

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安徽中医药大学第一附属医院风湿科,安徽 合肥 230031

新风胶囊(XFC) 环状RNA Cbl原癌基因B(circ-CBLB) 成纤维细胞样滑膜细胞(FLS) 细胞增殖 细胞凋亡 细胞因子

国家中医药管理局第七批全国老中医药专家学术经验继承项目国家自然科学基金面上项目国家自然科学基金面上项目安徽省高校优秀青年人才支持计划项目新安医学教育部重点实验室安徽省高校自然科学研究项目安徽省新时代育人质量工程项目

国中医药人教函[2022]76号8197365582274501皖教秘人[2022]11号2020xayx04KJ2020A03972022xscx103

2024

细胞与分子免疫学杂志
中国免疫学会,第四军医大学

细胞与分子免疫学杂志

CSTPCD北大核心
影响因子：0.817
ISSN：1007-8738
年,卷(期)：2024.40(9)