Mechanism of miR-200b-3p-induced FOSL2 inhibitorion of endometrial cancer cell proliferation and metastasis
Objective The purpose of this study was to investigate how miR-200b-3p inhibitors the proliferation and metastasis of endometrial cancer(EC)cells by inducing the expression of FOS-like antigen 2(FOSL2)of activator protein 1(AP1)transcription family.Methods Endometrial cancer cell line HEC-1-A was divided into 12 groups:NC-mimic(transfected with negative control NC mimic),miR-200b-3p mimic(transfected with miR-200b-3p mimic),NC-inhibitor(transfected with negative control NC inhibitor),miR-200b-3p inhibitor group(transfected with miR-200b-3p inhibitor),si-NC(transfected with negative control Si-NC),si-FOSL2(transfected with si-FOSL2),oe-NC(transfected with negative control oe-NC),oe-FOSL2 group(oe-FOSL2),miR-200b-3p mimic+oe-NC group(co-transfected with miR-200b-3p mimic and oe-NC),miR-200b-3p mimic+oe-FOSL2 group(co-transfected with miR-200b-3p mimic and oe-FOSL2),miR-200b-3p inhibitor+si-NC group(co-transfected with miR-200b-3p inhibitor and si-NC),miR-200b-3p inhibitor+si-FOSL2 group(co-transfected with miR-200b-3p inhibitor and si-FOSL2).Real-time fluorescence quantitative PCR,Western blot,CCK-8 assay,scratch test and Transwell assay were used to detect the expression of miR-200b-3p mRNA,FOSL2 mRNA and protein expression level,cell proliferation,migration and invasion.Results In endometrial cancer cell lines,the expression of miR-200b-3p was significantly down-regulated,while the expression of FOSL2 was significantly up-regulated.Compared with NC-mimic group,the expression of FOSL2,N-cadherin and Vimentin in miR-200b-3p mimic group was significantly decreased,and the expression of E-cadherin was significantly increased.The cell proliferation,migration rate and the number of transmembrane cells were significantly decreased.Compared with the miR-200b-3p mimic+oe-NC group,the expression of FOSL2,N-cadherin and Vimentin in miR-200b-3p mimic+oe-FOSL2 group was significantly increased,and the expression level of E-cadherin was significantly decreased,and the cell proliferation,migration rate and the number of transmembrane cells were significantly increased.Compared with NC-inhibitor group,the expression of FOSL2,N-cadherin and Vimentin in miR-200b-3p inhibitor group was significantly increased,and the expression of E-cadherin was significantly decreased.The cell proliferation,migration rate and the number of transmembrane cells were significantly increased.Compared with the miR-200b-3p inhibitor+si-NC group,the expression of FOSL2,N-cadherin and Vimentin in miR-200b-3p inhibitor+si-FOSL2 group was significantly decreased,and the expression of E-cadherin was significantly increased;the cell proliferation,migration rate and the number of transmembrane cells were significantly decreased.Conclusion The expression of miR-200b-3p in endometrial cancer cells is down-regulated,which can inhibitor the proliferation,migration and invasion of endometrial cancer cells by regulating the EMT process,and its mechanism is related to its targeted negative regulation of FOSL2 expression.
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