首页|A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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To understand the molecular mechanisms underlying chloroplast development, we isolated and characterized the albino mutant emb1303-1 in Arabidopsis. The mutant displayed a severe dwarf phenotype with small albino rosette leaves and short roots on a synthetic medium containing sucrose. It is pigment-deficient and seedling lethal when grown in soil. Embryo development was delayed in the mutant, although seed germination was not significantly im-paired. The plastids of emb1303-1 were arrested in early developmental stages without the classical stack of thylakoid membrane. Genetic and molecular analyses uncovered that the EMB1303 gene encodes a novel chloroplast-localized protein. Mieroarray and RT-PCR analyses revealed that a number of nuclear-and plastid-encoded genes involved in photosynthesis and chloroplast biogenesis were substantially downregulated in the mutant. Moreover, the accu-mulation of several major chloroplast proteins was severely compromised in emb1303-1. These results suggest that EMBI303 is essential for chloroplast development.

EMB 1303albinochloroplast developmentembryogenesisArabidopsis

Xiaozhen Huang、Xiaoyan Zhang、Shuhua Yang

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State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China

National Plant Gene Research Center, Beijing 100193, China

国家自然科学基金国家自然科学基金Ministry of Science and Technology of China教育部新世纪优秀人才支持计划Key Project of Chinese Ministry of Education

30670181307702022009CB 119103NCET-05-0124106037

2009

细胞研究(英文版)
中国科学院上海生化细胞所

细胞研究(英文版)

CSTPCDCSCDSCI
影响因子:1.355
ISSN:
年,卷(期):2009.(10)
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