首页|Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/ CIPK6 calcium sensor/protein kinase complex
Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/ CIPK6 calcium sensor/protein kinase complex
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Potassium (K+) channel function is fundamental to many physiological processes. However, components and mechanisms regulating the activity of plant K+ channels remain poorly understood. Here, we show that the calcium (Ca2+)sensor CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane (PM)targeting of the K+ channel AKT2 from Arabidopsis thaliana by mediating translocation of AKT2 to the PM in plant cells and enhancing AKT2 activity in oocytes. Accordingly, akt2, cbl4 and cipk6 mutants share similar developmental and delayed flowering pheuotypes. Moreover, the isolated regulatory C-terminal domain of CIPK6 is sufficient for mediating CBL4- and Ca2+-dependent channel translocation from the endoplasmic reticulum membrane to the PM by a novel targeting pathway that is dependent on dual lipid modifications of CBL4 by myristoylation and palmitoylation. Thus, we describe a critical mechanism of ion-channel regulation where a Ca2+ sensor modulates K+ channel activity by promoting a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the PM.
Institut fiir Biologie und Biotechnologie der Pflanzen, Universit(a)t Münster, 48149 Miinster, Germany
Biochimie et Physiologie Moléculaire des Plantes (UMR 5004 CNRS-INRA-SupAgro-UM2), Campus INRA-SupAgro, Place Viala, F34060 Montpellier Cedex 1, France
Institut für Biochemie und Biologie, Universit(a)t Potsdam, Karl-Liebkencht-Str. 24/25, 14476 Potsdam-Golm, Germany
This work was supported by grants from the DFG to IDThis work was supported by grants from the DFG to IDThis work was supported by grants from the DFG to IDto JKto JKHFSP to JKfrom the ANR Genoplante to CC-F,BL and J-BT
Heisenberg-fellowshipDFG DR 430/5-15-2SFB 629/B9AFGN Ku931/7-1RGP033/2006-CANR-06-GPLA-012
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