首页|Dicer-independent RNA-directed DNA methylation in Arabidopsis

Dicer-independent RNA-directed DNA methylation in Arabidopsis

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RNA-directed DNA methylation (RdDM) is an important de novo DNA methylation pathway in plants.Small interfering RNAs (siRNAs) generated by Dicers from RNA polymerase Ⅳ (Pol Ⅳ) transcripts are thought to guide sequence-specific DNA methylation.To gain insight into the mechanism of RdDM, we performed whole-genome bisulfite sequencing of a collection of Arabidopsis mutants, including plants deficient in Pol Ⅳ (nrpd1) or Dicer (dcl1/2/3/4) activity.Unexpectedly, of the RdDM target loci that required Pol Ⅳ and/or Pol Ⅴ, only 16％ were fully dependent on Dicer activity.DNA methylation was partly or completely independent of Dicer activity at the remaining Pol Ⅳ-and/ or Pol Ⅴ-dependent loci, despite the loss of 24-nt siRNAs.Instead, DNA methylation levels correlated with the accumulation of Pol Ⅳ-dependent 25-50 nt RNAs at most loci in Dicer mutant plants.Our results suggest that RdDM in plants is largely guided by a previously unappreciated class of Dicer-independent non-coding RNAs, and that siRNAs are required to maintain DNA methylation at only a subset of loci.

siRNAnon-coding RNAPol ⅣDicer-likeRdDMDNA methylation

Dong-Lei Yang、Guiping Zhang、Kai Tang、Jingwen Li、Lan Yang、Huan Huang、Heng Zhang

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Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095,China

Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA

Chinese Academy of Sciences and US National Institutes of Health

R01GM070795 to J-K Z

2016

10.1038/cr.2015.145

细胞研究(英文版)
中国科学院上海生化细胞所

细胞研究(英文版)

CSTPCDCSCDSCI
影响因子：1.355
ISSN：
年,卷(期)：2016.26(1)
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