Expression of miR-140-5p in breast cancer cell line MDA-MB-231 under hypoxia and its effect on proliferation,invasion and migration function
Objective This study aims to explore the effect of miR-140-5p on the function of breast cancer cells un-der hypoxia.Methods The breast cancer cell line MDA-MB-231 was routinely cultured and treated with hypoxia.The expression level of miRNA-140-5p in MDA-MB-231 cells under hypoxia and normoxia was detected by qt PCR.The pro-tein expression level of miRNA-140-5p in MDA-MB-231 cells under hypoxia and normoxia was detected by WB experi-ment.The cells were transfected with miRNA-140-5p mimics.The cells were divided into groups,and the cells in each group were proliferated Migration and invasion ability testing;Finally,detect the expression of NOX4 in each group of cells and evaluate the regulatory relationship between miR-140-5p and NOX4.Results The expression of miRNA-140-5p decreased in the hypoxic state of MDA-MB-231 cells(P＜0.05),while the expression of miRNA-140-5p mimics increased after transfection.miRNA-140-5p mimics could enhance the proliferation,migration,and invasion ability of cells in hy-poxic environments;miRNA-140-5p mimics could also enhance the expression of NOX4 in MDA-MB-231.Conclusion The expression of miRNA-140-5p in breast cancer cell MDA-MB-231 under hypoxia is reduced,and the expression of miRNA-140-5p is increased after transfection with Mimic,and it has an impact on the migration,invasion and prolifera-tion of MDA-MB-231 under hypoxia,indicating that the impact of miRNA-140-5p on the function of breast cancer cell MDA-MB-231 under hypoxia may lead to the occurrence and development of breast cancer.And this mechanism may be formed by inducing changes in NOX4 in MDA-MB-231.
万方数据
维普
