The clinical significance of miR-181a and ATG5 expression in breast cancer patients and their regulation on autophagy of MCF-7 cell line
Objective To investigate the expression of miR-181a and ATG5 in breast cancer patients,and further study their regulatory effects on autophagy of MCF-7 breast cancer cell line,in order to reveal their clinical significance and potential mechanism in the development of breast cancer.Methods In this study,120 patients with breast cancer ad-mitted from June 2021 to September 2022 were selected as disease group and 55 healthy subjects were selected as normal control group.The expression of miR-181a and ATG5 in serum and tissues was detected by qPCR.CCK-8 was used to detect the changes in the proliferation ability of MCF-7 cells,and Western blot was used to detect the expression of auto-phagy and apoptosis-related proteins in MCF-7 cells.The apoptosis rate of MCF-7 cells in each group was detected by flow cytometry.Results The expression of miR-181a in serum and tissues of breast cancer patients was significantly in-creased,while the expression of ATG5 was significantly decreased(P＜0.01).The expression of miR-181a was closely related to lymph node metastasis,TNM stage and tumor differentiation(P＜0.01).Inhibition of miR-181a expression could significantly reduce the proliferation capacity of MCF-7 cells,promote the expression of autophagy related proteins ATG5,LC3B and Beclin-1,and promote the expression of apoptotic BAX/BCL-2 protein ratio.Conclusion The up-reg-ulation of miR-181a is associated with the clinical characteristics of breast cancer and may be used as a potential marker for disease assessment.In addition,miR-181a may affect the proliferation and survival of breast cancer cells by regulating autophagy and apoptosis.It is expected to provide a new research direction and clinical application prospect for the diag-nosis and treatment of breast cancer.
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