Systematic analysis of expression profiles and prognostic significance for ARL family in stomach adenocarcinoma
Objective To research the expression of ARL family and its correlation with prognosis in stomach ade-nocarcinoma.Methods The gene expression and mRNA levels of ARL family in gastric cancer were extracted from TC-GA database,GTEx project,MSK cancer gene detection and so on;the relative and differential expression were analyzed using various databases above.Immunohistochemistry(IHC)was used to detect the protein expression of ARL9 and p53 in stomach adenocarcinoma.The clinical stage of gastric cancer from TCGA database and GTEx project was used to esti-mate the correlation between ARL family and clinical stage.The data of overall survival(OS)from GEO,EGA and TC-GA database were analyzed to assess the correlation between ARL family and OS.The samples of stomach adenocarcino-ma were used to detect the mutation of ARL family and the corresponding prognosis.The GeneOntology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to determine the co-expression and pathways of ARL family.Results The mRNAs levels of ARL1,ARL4A,ARL4C,ARL5A,ARL5B,ARL8A,ARL9,ARL11,ARL13A,ARL13B,ARL15,ARL16 and ARL18 were significantly increased in gastric cancer tissues;the mRNA levels of ARL4D was significantly decreased(P＜0.05).The IHC results showed that the protein expression of ARL9 and p53 was positive in gastric cancer tissues.The expression of ARL2 and ARL14 were closely related to clinical stages(P＜0.05).Higher mRNA expressions of ARL2,ARL3,ARL4D,ARL9,ARL13B,ARL16 and ARL18 mRNA were discovered to be neg-atively associated with OS in stomach adenocarcinoma,higher mRNA expressions ofARL1,ARL4A,ARL14 and ARL15 were positively associated with OS.The mutation of missense,splice,truncating,amplification and deep deletion was de-tected in stomach adenocarcinoma.The GO and KEGG analysis showed the co-expression of ARL family and participation in the cell signaling.Conclusion ARL family is significantly expressed in gastric cancer and expressively associated with prognosis.
万方数据
维普
