Quality evaluation of Euryale semen from different areas by HPLC fingerprint combined with chemometrics
Objective To establish a method for the quality evaluation of Euryale semen from different areas by fingerprint combined with chemometrics.Methods 33 samples were collected from 11 provinces.HPLC chromatograms were obtained.The common pattern was established for chemometrics analysis.Similarity analysis,2D hierarchical cluster analysis, principal component analysis were performed.Results Euryale semen from 11 provinces could be classified by common pattern which contained 9 characteristic peaks using similarity analysis.The similarity of Shandong,Hunan,Jiangxi,Anhui and Hebei provinces were over 0.90,while the similarity of other areas were less than 0.90.It showed the significant differences between samples.No.1,3,4 peaks were the most important peaks which could be used to identify the differences between areas by hot maps of hierarchical cluster analysis and loading chart of principal component analysis.Samples from Jiangsu province were more stable than from other areas.Conclusion This method is flexible and comprehensive,and could differentiate and evaluate Euryale semen of different areas without reference standard.It could be used to control the quality of Euryale semen.
NETL
NSTL
万方数据
维普
