Anti-tumor activity of Juglans mandshurica water extract with or without juglone
Objective Juglans mandshurica (JM) extract and juglone,one major compound of JM, both showed anti-tumor activity.This paper was aimed to explore the contribution that juglone made to the anti-tumor activity of JM extract.Methods According to the evaporability of juglone,it was knocked out of JM extract by decocting,and the JM water extract without jouglone was prepared.By adding juglone to the JM decoction,JM water extract with juglone was obtained.The in vivo anti-tumor activity of JM water extract with or without juglone was compared by mice bearing murine-hepatocarcinoma (H22) model.Tumor weight and tumor inhibition ratio were obtained for the evaluation of the anti-tumor activity.Immune organ index and peripheral blood parameters were determined to estimate the protective effects on normal functions of H22 tumor bearing mouse.Results JM water extract with juglone showed anti-tumor activity in a dose-dependent manner,with tumor inhibition ratio of 31.96% and 50.61%,at low and high dose,respectively.While the tumor inhibition ratio of JM water extract without juglone at low and high dose was 37.01% and 12.35%,respectively.At high dose,the anti-tumor activity of JM water extract with juglone was statistically stronger than that without juglone.JM water extract with juglone at low dose could rectify the number of red blood cell of mice bearing H22 tumor,and JM water extract without juglone at high dose could adjust the abnormal of the percentage of lymphocyte and neutrophil to normal state.Other JM water extract showed no effect on the immune organs and peripheral blood parameters.Conclusion JM water extract exhibited potent anti-tumor activity.At high dose, juglone made major contribution to the anti-tumor activity of JM water extract.
Juglans mandshuricajuglonewater extractH22 murine-hepatocarcinomain vivo anti-tumor activity
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万方数据
维普
