苦瓜中葫芦烷型三萜成分及其抗肝纤维化活性的研究
Cucurbitane triterpenoids from Momordica charantia L.and their anti-hepatic fibrosis activity
岳佳音 1徐静 2张晓书 2赵余庆2
作者信息
- 1. 沈阳医学院药学院,沈阳 110034
- 2. 沈阳药科大学功能食品与葡萄酒学院,沈阳 110016
- 折叠
摘要
目的 研究苦瓜酸水解产物的化学成分及其抗肝纤维化活性.方法 用正、反相柱层析等色谱手段进行分离纯化,用波谱技术与文献数据对照鉴定其结构;并用四甲基偶氮唑盐(methylthiazolyl tetrazolium,MTT)法测定部分化合物体外对大鼠肝星状细胞(t-HSC/Cl-6)增殖的抑制作用.结果 共分离出 10个葫芦烷型三萜类化合物,分别鉴定为:5β,19-epoxy-3β-hydroxy-19(R)-methoxy-27-norcucurbita-6,23(E)-dien-25-one(1);5β,19-epoxy-3β-hydroxy-19(S)-methoxy-27-norcucurbita-6,23(E)-dien-25-one(2);5β,19-epoxy-25-methoxycucurbita-6,23(E)-dien-3β-ol(3);karavilagenin A(4);(23E)-5β,19-epoxycucurbita-6,23,25-triene-3β-ol(5);23E-5β,19-epoxy-cucurbita-6,23-dien-3β,25-diol(6);25-O-methylkaravilagein D(7);(23E)-3β-hydroxy-7β,25-dimethoxycucurbita-5,23-dien-19-al(8);karavilagenin B(9);(19S,23E)-5β,19-epoxy-19-methoxycucurbita-6,23-diene-3β,25-diol(10).体外活性筛选发现化合物1和化合物2对t-HSC/Cl-6有一定的抑制细胞增殖作用,IC50 分别为(25.92±2.63)、(26.22±2.77)μmol·L-1.结论 化合物1和化合物2为首次从该植物中分离得到,其对肝纤维化细胞增殖有一定的抑制作用.
Abstract
Objective To investigate the chemical constituents from acid hydrolyzed ethanol extract of Momordica charantia L.and their anti-hepatic fibrosis activity.Methods The compounds were isolated and purified by various chromatographic techniques,and their structures were identified by spectroscopic analysis and spectral data.Methylthiazolyl tetrazolium(MTT)assay was used to de-termine the cytotoxic activity of some compounds against activated rat hepatic stellate cells(t-HSC/Cl-6)in vitro.Results 10 cucur-bitane triterpenoids were isolated from the acid hydrolyzed ethanol extract of M.charantia.Their structures were identified as 5β,19-epoxy-3β-hydroxy-19(R)-methoxy-27-norcucurbita-6,23(E)-dien-25-one(1);5β,19-epoxy-3β-hydroxy-19(S)-methoxy-27-nor-cucurbita-6,23(E)-dien-25-one(2);5β,19-epoxy-25-methoxycucurbita-6,23(E)-dien-3β-ol(3);karavilagenin A(4);(23E)-5β,19-epoxycucurbita-6,23,25-triene-3β-ol(5);23E-5β,19-epoxy-cucurbita-6,23-dien-3β,25-diol(6);25-O-methylkaravilagein D(7);(23E)-3β-hydroxy-7β,25-dimethoxycucurbita-5,23-dien-19-al(8);karavilagenin B(9);and(19S,23E)-5β,19-epoxy-19-methoxycucurbita-6,23-diene-3β,25-diol(10),respectively.Compounds 1 and 2 showed moderate cytotoxicity against the hepatic stellate cells tested in vitro with IC50 values of(25.92±2.63)and(26.22±2.77)μmol·L-1 respectively.Conclusion Compounds 1 and 2 were isolated from the fruit of M.charantia for the first time,and they can inhibit the pro-liferation of hepatic fibrotic cells.
关键词
苦瓜/葫芦烷型三萜化合物/抗肝纤维化活性Key words
Momordica charantia L./cucurbitane triterpenoids/anti-hepatic fibrosis activity引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据