Cucurbitane triterpenoids from Momordica charantia L.and their anti-hepatic fibrosis activity
Objective To investigate the chemical constituents from acid hydrolyzed ethanol extract of Momordica charantia L.and their anti-hepatic fibrosis activity.Methods The compounds were isolated and purified by various chromatographic techniques,and their structures were identified by spectroscopic analysis and spectral data.Methylthiazolyl tetrazolium(MTT)assay was used to de-termine the cytotoxic activity of some compounds against activated rat hepatic stellate cells(t-HSC/Cl-6)in vitro.Results 10 cucur-bitane triterpenoids were isolated from the acid hydrolyzed ethanol extract of M.charantia.Their structures were identified as 5β,19-epoxy-3β-hydroxy-19(R)-methoxy-27-norcucurbita-6,23(E)-dien-25-one(1);5β,19-epoxy-3β-hydroxy-19(S)-methoxy-27-nor-cucurbita-6,23(E)-dien-25-one(2);5β,19-epoxy-25-methoxycucurbita-6,23(E)-dien-3β-ol(3);karavilagenin A(4);(23E)-5β,19-epoxycucurbita-6,23,25-triene-3β-ol(5);23E-5β,19-epoxy-cucurbita-6,23-dien-3β,25-diol(6);25-O-methylkaravilagein D(7);(23E)-3β-hydroxy-7β,25-dimethoxycucurbita-5,23-dien-19-al(8);karavilagenin B(9);and(19S,23E)-5β,19-epoxy-19-methoxycucurbita-6,23-diene-3β,25-diol(10),respectively.Compounds 1 and 2 showed moderate cytotoxicity against the hepatic stellate cells tested in vitro with IC50 values of(25.92±2.63)and(26.22±2.77)μmol·L-1 respectively.Conclusion Compounds 1 and 2 were isolated from the fruit of M.charantia for the first time,and they can inhibit the pro-liferation of hepatic fibrotic cells.
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