Protective effect of breviscapine on myocardial ischemia-reperfusion injury in rats
Objective To observe the myocardial protective effect of breviscapine on myocardial ischemia-reperfusion injury(MIRI)in rats,and to explore the possible mechanism.Methods Sixty SD rats were randomly divided into healthy group,model group,low-dose and high-dose groups of breviscapine,15 rats in each.The rats in the low and high dose groups of breviscapine were intraperito-neally injected with breviscapine(50,100 mg·kg-1).Rats in the healthy group and model group were intraperitoneally injected with an equal volume of normal saline.Once a day,the intervention lasted for 5 days.Ten animals were included in the model group,low-dose and high-dose groups of breviscapine.The electrocardiogram,serum myocardial injury indicators,serum oxidative stress indica-tors,and the serum inflammatory factors were detected.The myocardial infarct size was detected by 2,3,5-triphenyltetrazolium chlo-rid(TTC)staining.The protein expressions of interleukin-23(IL-23)and interleukin-17(IL-17)in myocardial tissue was detected by Western blotting method.Results Compared with the MIRI group,the number of the occurrences of ventricular fibrillation(VF),ventricular tachycardia(VT),serum creatine kinase isoenzyme(CK-MB)activity,cardiac troponin T(cTnT)activity,malondial-dehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)levels,myocardial tissue IL-23,IL-17 protein expre-ssion were decreased,the VF and VT continued time were reduced,and the serum superoxide dismutase(SOD)activity was in-creased in the low-dose group of breviscapine(P＜0.05).Low-dose group of breviscapine and high dose group of breviscapine had the same changes in various indices,and high dose group of breviscapine was more significant(P＜0.05).Conclusion Breviscapine can relieve arrhythmia,reduce myocardial damage,oxidative stress and inflammation in MIRI rats,and its mechanism of action may be related to the inhibition of IL-23/IL-17 axis activity.
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万方数据
维普
