Mechanism of ligustrazine regulating cisplatin resistance in ovarian cancer cells based on KDM2B
Objective To explore the potential mechanism of ligustrazine regulating cisplatin resistance in ovarian cancer cells.Methods Cisplatin(DDP)was used to induce A2780 to become A2780 cisplatin-resistant cell lines(A2780/DDP).This study was divided into control group,negative control group,interference group,and ligustrazine group.The interference group and the negative control group were transfected with si-KDM2B and si-NC,respectively.The ligustrazine group was given a final concentration of 5 nmol∙L-1 ligustrazine(dissolved in the medium)to treat the cells,and the control group was given the same volume of the medium.After each group of cells were cultured for 48 hours,the drug-resistant cells were collected.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect mRNA expression levels,methyl thiazolyl tetrazolium(MTT)was used for exploring cell proliferation,flow cytometry was carried out for detecting apoptosis,and Wes-tern blotting was used for detecting the expression levels of apoptosis-related proteins[B-cell lymphoma-2(BCl-2),BCL2-as-sociated X protein(Bax)and caspase-3].Results Lysine-specific demethylase 2B(KDM2B)was highly expressed in A2780/DDP.After treated with ligustrazine or knockdown of KDM2B,A2780/DDP cells inhibited cell proliferation,promoted cell apoptosis,up-regulated apoptosis-related proteins(Bax and caspase-3)and down-regulated BCl-2 expression level in A2780/DDP.Conclusion Ligustrazine may inhibit A2780/DDP cell proliferation and promote cell apoptosis by KDM2B.
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