Objective To explore the potential mechanism of ligustrazine regulating cisplatin resistance in ovarian cancer cells.Methods Cisplatin(DDP)was used to induce A2780 to become A2780 cisplatin-resistant cell lines(A2780/DDP).This study was divided into control group,negative control group,interference group,and ligustrazine group.The interference group and the negative control group were transfected with si-KDM2B and si-NC,respectively.The ligustrazine group was given a final concentration of 5 nmol∙L-1 ligustrazine(dissolved in the medium)to treat the cells,and the control group was given the same volume of the medium.After each group of cells were cultured for 48 hours,the drug-resistant cells were collected.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect mRNA expression levels,methyl thiazolyl tetrazolium(MTT)was used for exploring cell proliferation,flow cytometry was carried out for detecting apoptosis,and Wes-tern blotting was used for detecting the expression levels of apoptosis-related proteins[B-cell lymphoma-2(BCl-2),BCL2-as-sociated X protein(Bax)and caspase-3].Results Lysine-specific demethylase 2B(KDM2B)was highly expressed in A2780/DDP.After treated with ligustrazine or knockdown of KDM2B,A2780/DDP cells inhibited cell proliferation,promoted cell apoptosis,up-regulated apoptosis-related proteins(Bax and caspase-3)and down-regulated BCl-2 expression level in A2780/DDP.Conclusion Ligustrazine may inhibit A2780/DDP cell proliferation and promote cell apoptosis by KDM2B.
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