Effects of baicalin on proliferation and apoptosis of granulosa cells in rats with polycystic ovary syndrome
Objective To investigate the effects of baicalin on the proliferation and apoptosis of granulosa cells in rats with polycystic ovary syndrome(PCOS),and the regulation of phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt)signaling pathway.Methods Forty-eight rats were successfully established PCOS model and randomly divided into model group,baicalin low-dose group,baicalin high-dose group and positive control group,12 rats in each group.Another 12 rats were selected as control group.Ba-icalin low-dose and baicalin high-dose groups were given baicalin 25 mg∙kg-1 and 50 mg∙kg-1,respectively.The positive control group was given Ethinylestradiol and Cyproterone Acetate Tablets(Diane-35)0.3392 mg∙kg-1.The control group and model group were given equal volume of normal saline once a day,for consecutive 3 weeks.The contents of luteinizing hormone(LH),follicle stimulating hormone(FSH),testosterone(T)and estradiol(E2)were detected by enzyme linked immunosorbent assay(ELISA),the pathological damage of ovarian tissue was observed by HE staining,the proliferation rate of granulosa cells was detected by CCK-8,the apoptosis rate was detected by flow cytometry,the relative expression levels of PI3K,p-PI3K,Akt and p-Akt were detected by Western blotting.Results In the control group,luteum was observed,but expanded follicles were not observed.In the model group,the ovarian volume was enlarged,luteum was rare,and cystic dilated follicles were visible.In the low and high dose baicalin groups and the positive control group,the ovarian volume was reduced,luteum was visible,and cystic follicle amplification was not obvious.Compared with control group,the levels of FSH and E2 and the proliferation rate of granulosa cells and the relative expression of p-PI3K and p-Akt protein in model group were decreased,while the levels of LH and T,the apoptosis rate of granulosa cells were in-creased(P＜0.05).Compared with model group,the levels of FSH and E2 and the proliferation rate of granulosa cells and relative ex-pression of p-PI3K and p-Akt protein were increased in baicalin low-dose and high-dose groups and positive control group,while the levels of LH and T,apoptosis rate of granulosa cells decreased,and the changes of all indicators in positive control group were the most significantly.The second group was baicalin high-dose group and baicalin low-dose group(P＜0.05).Conclusion Baicalin can promote the proliferation of ovarian granulosa cells in PCOS rats,inhibit apoptosis and improve pathological damage of ovarian tissue,possibly by inhibiting PI3K/Akt signaling pathway.
baicalinpolycystic ovary syndromegranulosa cellsproliferationapoptosisphosphatidylinositol-3-kinase/protein ki-nase B signaling pathway
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万方数据
维普
